Artemisia Sphaerocephala AsFer Gene Transformation Of Tobacco And Drought Resistance Research

Artemisia sphaerocephala, the endemic plant in desert and semi-desert region, is an important dominant species in the sandy vegetation species. Ferritin, widely exists in the organism, is a kind of protein with antioxidant function. In this study, Artemisia sphaerocephala ferritin gene (AsFer) expression vector, pCAMBlA1301-AsFer, was constructed, and transgenic tobacco was obtained by agrobacterium mediated method. Finally, using transgenic tobacco and Artemisia sphaerocephala as materials, the drought resistance and the physiological changes of the plant under drought stress were studied.The main results are as follows:1. Expression vector, pCAMBIA1301-AsFer, was constructed, introduced into competent of Agrobacterium tumefaciens GV3301using freeze-thaw method, and finally introduced into tobacco NC89. Transgenic plants were screened through tissue culture and resistance screening.2. Drought stress test showed that the relative conductivity, content of MDA, proline, oxygen free radicals and hydrogen peroxide was significantly (P<0.05) lower in transgenic tobacco than that of wild-type tobacco, which indicated that transgenic tobacco with overexpression of AsFer suffered lighter oxidative damage compared to wild-type tobacco.3. When Artemisia sphaerocephala suffered drought stress, the plant conductivity, contents of proline, malondialdehyde, oxygen free radicals, and hydrogen peroxide showed an upward trend in the first6days and reaches maximum values and significantly higher than the control at6d(P <0.05); After rehydration, these indicators returned to control levels slowly. Ferritin expression gradual declined during the first4days drought stress, and then rapidly increased to a maximum at6d; after rehydration, ferritin levels returned to control level.