Effect Of PVP Coated ZnO Nanoparticles On The Mitosis Of Garlic Root Tip Cells

Nanotechnology is solving many of today’s problems in medicine, energy production, and environmental sustainability, due to the unique properties that many particles possess when manufactured at the nanometer scale. The widespread use of nanotechnology, therefore, is inevitable and will increase rapidly in the near future. Metal oxides, including zinc oxide (ZnO), are a class of manufactured nanoparticles (NPs) that are among the first nanoscale materials to be used in commercial and industrial products. Because of its semiconducting, optical, ultraviolet (UV)-shielding properties, ZnO NPs have been used in a large variety of commercial products such as cosmetics, sunscreens, electronics devices and chemical sensors, and has resulted in a growing public debate on the toxicological and environmental effects of direct and indirect exposure to these materials. According to recent toxicological study, ZnO NPs has potential toxicity of cells, plants, animals and ecologies. Although, studies on the toxicity of ZnO NPs are growing fast, ZnO NPs’ toxic mechanism is still unclear, and there is no consolidated solution to its toxicity. Therefore, in order to improve the understanding about the mechanism of ZnO NPs’ toxicity, we studied the effect of PVP coated ZnO NPs on the mitosis of garlic root tip cells.This work mainly studied the effect of PVP modification on the ZnO NPs’ toxicity. Different levels of PVP solution were used for the surface modification of ZnO NPs and the concentration of the ZnO NPs’ suspension was two times the fifty percent inhibitory concentration (IC(50)). The coated and uncoated ZnO NPs’ suspensions were then used in the cultivation of garlic. The results showed that surface modification with0.25mg/ml or higher concentrations of PVP suppressed the toxicity of ZnO NPs on the growth of garlic root and the mitosis of root tip cells in some degree. To the mitosis of root tip cells after18h and24h of incubation, the toxicity of ZnO NPs modified with higher concentrations of PVP (0.75mg/ml and1.0mg/ml) were suppress remarkably. This was statistically significant at P<0.01. For influence on the chromosome aberration rate, pure ZnO NPs were obviously negative. Although, there are some difference in the toxicity of pure ZnO Nps and surface coated ZnO NPs on the chromosome aberration rate, the difference were more complex and depended on the concentration of PVP and the cultivation time...