Antioxidative Activity And Safety Assessment Of Lactobacillus Plantarum C88

Eleven Lactobacillus plantarum strains isolated from traditional Chinese fermented foods were investigated for the in vitro scavenging activity against hydroxyl and DPPH radicals, and their resistance to hydrogen peroxide. All the strains showed different degrees of in vitro antioxidant activity. L. plantarum C88at a dose of1010CFU/ml showed the highest hydroxyl radical and DPPH scavenging activities with an inhibition rate of44.31%and53.05%, respectively. Resistance of intact cells to hydrogen peroxide was also found in all strains, and L.plantarum C88was the most resistant strain against hydrogen peroxide.The antioxidant activity of L. plantarum C88was further evaluated on H2O2induced Caco-2cell injury. Compared with the oxidative stress group, L. plantarum C88in1011CFU/ml significantly increased the glutathione peroxidase (GSH-Px)(P<0.01) and the total antioxidation capacity (T-AOC)(P<0.01) in the cultured supernatants, the hydroxyl radical scavenging ability (P<0.01), GSH-Px activity(P<0.01) and the superoxide dismutase (SOD) activity in the cell disruptions at24h. Our results suggested that L. plantarum C88could protect the Caco-2cells from H2O2-induced oxidative injury by renewing the enzymatic and non-enzymatic antioxidant defense system.L. plantarum C88was also subjected to in vivo antioxidant studies by administration to oxidative stress senescent mice induced by D-galactose. The in vivo results showed that administration with L. plantarum C88increased significantly the serum SOD activity, the activity of GSH-Px and the T-AOC in liver, and decreased significantly the level of malondialdehyde in liver of D-galactose treated mice. Overall, the present study indicates that L. plantarum C88with both in vitro and in vivo antioxidant activities, could be considered as a potential antioxidant strain to be applied in functional foods.The acute oral toxicity and bacterial translocation of L. plantarum C88were studied in mice to provide evidence for safety application. Different doses of L. plantarum C88were given to mice orally by gavage for14consecutive days. Compared with the control, there was not significant differences (P>0.05) in general signs, organs index, or biochemistry parameters of serum and liver with different doses of L. plantarum C88. No treatment-associated bacterial translocation was detected in any groups.