Research Of Human’s And Mouse’s MicroRNA-Target Structure And Conservative Of Target

MicroRNAs are one kind of non coding,they are functional small-molecule andcontain about21-25nucleotides.Their precursor are always in the intro position of gene.Theprecursor always has a secondary-structure consist of a sequence of stems and a hairpin.Bythe effort of the cut-enzyme,the secondary-structure will be cut and then product twosingle-stranded RNA,one of them or all of them may be the mature micro-RNAs. MicroRNAsis rich in eucaryons, and they have a very high conservative in species.In the previousresearch,microRNAs had the effort that make mRNA degradation or restrain mRNA’s codingeffort through the combination between microRNA’s seed region(the5’region of microRNA’s1-8nucleotides) and3’ untranslated region (UTR) of mRNA.So microRNAs play animportant role in the development and regulatory network of organisms.Although microRNAs only have21-25nt,the microRNA-Targets have some simplestructure. In the recent research,the microRNA-Target have more than seed model.In seedregion,there are some wobbles(the match between G and U) and bulges(the region that don’tproduct match),the bulge is divided to duplex bulge and single bulge.Except seed region,the3’ region and center region of microRNAs can also product match to mRNA.So it willproduct some structures among these regions.Some papers indicate that3’region and centerregion matchs are the remedy of seed region’s instability.Some experiments indicate that themix of combination model will product higher effort to the targets. The seed regions havebeen demonstrated conservative in evolution, but other region’s conservative have not beendemonstrated. Now there are a lot of databases have a abundant of microRNA-Target data thatthe combination has been demonstrated by papers and provide these paper in the same time.This paper will classify and profile these data by their combination model and structure,andthen get the same characters of these data.Now, human’s and mouse’s gene mapping have been completely sequencing out, Thescientists found that human and mouse genes of more than95%are quite similar, Both genehave quite high homology. The development and behavior between human and mouse havehigh similarity. Mouse body can also generate the same disease types and status, Even, mouse also caused psychological state by the pressure.If you want to understand microRNA inhuman body and the mechanism of action of some biological pharmaceutical action, you canmade it in mouse’s body. This paper aims to study the microRNA’s target structure datathrough paper like combination model,wobble’s count,bulge’s position and so on.Then I willpredict the combination between microRNA and mouse’s same gene if they have hignconservative rate.Because of microRNA in humans and mouse are the homologous，theexperiment of human aimed to demonstrate the effort of microRNA and effect of somedeseases and biological pharmaceutical can be made in mouse.This can also provide somenew structure and conservative characteristics to microRNA prediction.