Research On Biological Oxidation Of4-methyl Cyciohexanone

The oxidation of4-methyl cyclohexanone was firstly detected by CHMO and PAMO.Original PAMO had no oxidation effect.After making two point mutations of PAMO,Gln93Asp/Pro94Asp,the mutant was able to catalyze the oxidation of4-methyl-cyclohexanone, throguh the catalytic activity is rather low.CHMO had relatively high catalytic activity towads4-methyl cyclohexanone,which was selected for our following research.Enzymatic activity of CHMO was increased about26times through self-induced method.The choice of ISPR was used for solving the supression problem of substrate and product in the oxdiation process. By comparison of nine kinds of resins, SD600was selected for the following research, for the simple reason it had a stong adsorption to4-methyl cyclohexanone.Comparing the proportion of resin and the substrate to be added, the ratio of1:2was the best.In this case, the concentration of the substate was just below the inhibition level so that the reaction could be conducted at a faster rate. By means of SFPR, the concentration of the substrate could be improved.At the concentration of50mM, the conversion reached about60%, while the conversion was only6%without ISPR.ISPR was a effective way to improve the concentration of substrate, and it will have broad prospects for industrial application in the future.