| In order to adapt to the complex environment and resist against pathogens fromenvironment, plants form a variety of defense mechanisms themselves in thelong-term process. One of the most important mechanisms is SAR. In this study, wefocused our attention on NPR-like genes in SAR, what was important in cultivatingnew kinds of disease resistance of maize. In this experiment, by using maize genomedatabase resources and technology of reverse transcription, we found a NPR-like genein maize. The main results about this gene’s characteristics and functions were asfollows:1. The length of this gene was1434bp, encoding477amino acid. Logging onthe web site of NCBI and analyzing the sequence by Blast program, we found that oursequence was99%similar to maize NPR1（accession number: NM001154115）,while their amino acid sequences were at the same. So we named this gene asZmNPR1. Its molecular weight is5.68kDa, and the isoelectric point is53303.10;2. On the phylogenetic tree, ZmNPR1was high homology with rice OsNPR2,and its protein sequence mainly contained a POZ/BTB site and an ankyrin repeatdomain.3. Using the gene gun, we sent the ZmNPR1G into onion epidermal cells. Theresult showed that ZmNPR1mainly expressed in the nucleus.4. By using semi-quantitative PCR, we analyzed the profile of the ZmNPR1gene and PAL gene （one kind of genes encoding a pathogenesis-related protein）expressions by four kinds of treatments. According to the profile, we found that SAand pathogens could induce ZmNPR1expression, while MeJA suppressed gene’sexpression. Such change phenomenon was as same as PAL gene’s.5. Then, we built the plant overexpression vector and RNA interference vectorto transform maize plant. At last,19RNAi transgenic plants were successfully got.The results of PCR detections by ten plants were all positive.6. By HPLC, we analyzed the different between transgenic plants andwild-type plants about SA content. The result showed that the content of SA decline51%after ZmNPR1was interfered. |
PDF Full Text Request