Molecular Phylogeny Of The Drosophila Auraria Species Complex And The Temporal And Spatial Expression And Functional Prediction Of Gene CG33465、CG8399、CG8841in Drosophila Melanogaster

Drosophila melanogaster as one of the most classical model organisms has been supplied information for evolutionary biology, developmental biology, neuroscience and human disease research. Drosophila is a very powerful model for neurodegenerative diseases. Some information from phylogenetic analysis give clues to learn the evolutionary history of the biology, and some disease moles of Drosophila lead authors to new research area and new ideal. It will continue to be an ideal model organism and help us to understand the basic biological and human disease mechanisms greatly in the future.auraria species complex consists of five sibling species D.auraria、D.biauraria、 D.triauraria、D.quadraria、D.subauraria.Here we present new data derived from32genes to reconstruct the phylogeny of the Drosophila auraria species complex. A variety of statistical tests, as well as maximum likelihood mapping analysis, were performed to estimate data quality, suggesting that all genes had a high degree of contribution to resolve the phylogeny.Individual locus was analyzed using maximum likelihood (ML), and the concatenated dataset were analyzed using partitioned maximum likelihood (ML) and Bayesian analyses. Separated analysis produced various phylogenetic relationships, however, phylogenetic topologies from ML and Bayesian analysis based on concatenated dataset, at the subgroup level, were completely identical to each other with high levels of support. Our results recovered:D. triauraria branched off first whitin Drosophila auraria species complex, thereafter D.quadraria branched off, D.auraria and D.biauraria were the last. Suggesting that all genes had a high degree of contribution to resolve the phylogeny.And we use the temporal and spatial expression and P elemen gene knock out to study the function of gene CG33465、CG8399、CG8841in Drosophila melanogaster.Five different development stage and three expression part were choosen,there are egg,larve one,larve three,puple,adult;adult’s head,adult’pereion,adult’pleon.Our results recovered:CG33465may have a serine-type endopeptidase activity.