Study Of The Role Of Gleâ…  In The Heart Development Of Zebrafish

The heart is one of the first organs to form during the vertebrate organofaction,providing the strength to circulatory system. As it is known to us that there are manyfactors involved in the heart development, so the pathogenesis is complicated. In therecent years, new research focus has been continued in the cardiovascular system area:from protein to nucleic acid and from the ionchannel genes to the non-ionchannel genes.Thus the scope of research is wider and the level of research is deeper.In2008, a new and important non-ionchannel gene NUP155was found, impressingpeople of the pathogenesis related to atrial fibrillation. NUP155played an important rolein the structure and the function of nuclear pore complex. Researchers proved theevidences that NUP155and Gleâ…  could interact with each other in the experiment of thegenome-wide yeast two-hybrid screen.In the present research, zebrafish was used as a model animal to identify the roles ofzGleâ…  gene in early heart development. The zGleâ… , which is located in chromosome8,expressed ubiquitously. In this study, we successfully identified the expression pattern ofzGleâ…  through whole-mount in situ hybridization to zebrafish embryos. The abnormalheart was observed in zGleâ…  morphants through the injection of zGleâ… -MO. It was alsofound that the spaw and ins, genes which are related to the regulation of left-rightasymmetry and located in the lateral plate mesoderm, were predominantly influenced inzGleâ…  morphants.These results indicated that zGleâ…  played an essential role in early zebrafish heartdevelopment. The zGleâ…  morphants showed an interesting characteristic by theperturbation of left-right patterning involving the spaw and ins, thus leading to thedefective heart.Thus, we hope that present findings could contribute in clear understanding of the development and pathogenesis of the heart in higher vertebrates.