| DNA replication is one of the basic functions of the cells during growth anddevelopment.The chromosomes must be accurately copied and assigned to the next generationof cells. Therefore, the replication process is subject to very strict regulation.Any loss ofcontrol will lead to serious consequences, including developmental abnormalities, geneticdiseases, cancer. Eukaryotic origin of replication or replicon is an important aspect of the copyarea of research.Yeast is a class of lower eukaryotes, not only has the growth characteristics similar toprokaryotes, with general eukaryotic molecular biology and cell biology properties, as a studyto determine replication initiation sites or replicon materials. Yeast chromosome III is the firstcompleted sequencing of the present study the clearest Autonomous replication sequence ARS(autonomously replicating sequence) is transformed into yeast cell’s independent replication ofa DNA sequence integrated into the yeast chromosome, as the yeast replication initiation site.ARS305is a high activity in Saccharomyces cerevisiae chromosome III, a highfrequency of use of the replicon. In order to study the impact of both sides of the sequence ofits activity, we constructed a series of recombinant plasmid pRS405as a carrier to ARS305inserts different lengths as the core sequence different on both sides; the recombinant plasmidusing of PEG/LiAc transformation of brewing wine yeast YPH499competent cells. Foridentify the sequence of ARS305both sides of the length of its replication activity, it has beenmeasured the conversion rate of the recombinant plasmid. The results showed that: ARS305both sides of the sequence length are longer, its autonomous replication activity of the higher.In this study, the sequence of ARS on both sides to form a nucleosome ability, ARSactivity and nucleosome positioning has made a preliminary exploration for study moredeeply. |
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