Bioinformatic Analysis Of The Elaeagnaceae NrDNA ITS Sequences

With the development of the human genome project a lot of species gene have been sequenced reduced biological data resources been explosive growth. Nowadays a lot of Elaeagnaceae genes is sequenced. Rich data resources for digging biological information created the conditions. Based on Network resource platforms50Elaeagnaceae nrDNA ITS sequences were obtained from the GenBank nucleotide database which including33Hippophae and5Elaeagnus also download12E. moorcroftii ITS sequences. Bioinformatic Analysis of the Elaeagnaceae nrDNA ITS Sequences.The main results are as follows.1. Bioinformatics analysis of Elaeagnaceae nrDNA ITS sequencesElaeagnaceae of ITS sequence5.8S segment is the most conservative, ITS2is the second smallest, and ITS1is biggest change. The5.8S segment bases mutation stransition more than transversion.34and160bases of H. tibetana for cytosine (C) and guanine (G) are different from other seabuckthorn species;130and133bases of the Hippophae existing for adenine (A) and cytosine (C) different from Elaeagnus;58and121bases in Sect.Hippophae are different from Sect. Gyantsenses which mutations can be used as a difference between and among the sites of information.The average GC content (56.1%) of Sect.Gyantsenses is slightly higher than the Sect.Hippophae (54.8%) and the average GC content of H.tibetana is higher than other species found that as the rise of altitude species GC content a trend of increase.Hippophae of5.8S RNA secondary structure similar but there exist great differences between Hippophae have two conformations of secondary structure I and II. Elaeagnus of5.8S RNA secondary structure for conformation I.Most of Hippophae5.8S RNA secondary structure for conformation II. According to the branch ring and the difference of issuing ring, divided conformation into IIA; ⅡB and IIC. H. tibetana (ⅡC) secondary structure is clearly distinguished from other Hippophae which reflecting that the H. tibetana is one of the independent evolution specie and support that H. tibetana lineages differentiation. Preliminary speculated that5.8S secondary structure huge "transform" sequence is pseudogene. Through the analysis of the GC content of the5.8S sequence,detection of methylated sites,5.8S motif, and secondary structure stability to speculate ITS pseudogene sequences which including ΨFN687766,ΨAF440247,ΨAF440251, ΨFN687767, ΨFN687767, ΨFN687769and ΨAF440258.Hippophae are all diploid (2n=24) plants but the NOR have different reports reported the number of position is not all the same. Perhaps with the generation of hybrid and chromosome restructuring that make ITS with evolution to slowly and gradually formed ITS pseudogenes.The pseudogene exception of the branch length when Using5.8S fragment phylogenetic analysis which have the same kind of individual sequences clustering together does not affect the structure of the phylogenetic tree. The phylogenetic seperate Sect.Hippophae and Sect. Gyantsenses partly and also support a monophyletic origin of Hippophae. The pseudogenes in the base of phylogenetic tree which using5.8S secondary structure to construct and separated from the functional sequences.The secondery structures can serve as the new source of information in Hippophae molecular systematic.2. Bioinformatic analysis of E. Moorcroftii nrDHA ITS sequencesThree parts of E. moorcroftii ITS sequences bases mutations the transition higher than transversion and the GC content is higher than AT content. ITS2information sites is the most abundant.5.8S is most conservative.E. moorcroftii ITS1is composed of three main base pairing of the double helix structure (stem) multi-branch ring. ITS2congormation is the ring of the four stems of multi-branch. Through the analysis of the5.8S segment and secondary structure predict ITS pseudogenes(ΨEF423354).The phylogenetic tree of the E. moorcroftii did not completely cluster same region together where Gansu and Xinjiang were clustered together which may as introduction about people frequently.