The Study Of Coupling Effect Between CaV1.2α1and BK Channel

L-type voltage-gated calcium channels（L-VGCCs）， one of the main ways inducingCa²⁺influx，are the target of many kinds of neurotransmitter，hormone and drugs. BKchanne(lLarge-conductance voltage-and Ca²⁺-activated K+channels)extensively exists inexcitable cells, especially in neural system, which has a significant physiological fuctionin mediating the concentration of intracellular calcium ions and the membrane potential，plays a crucial role in modulating a number of important physiological processes,such assmooth muscle contraction, neuronal excitability, frequency tuning of hair cells andimmunity.L-VGCCs and BK channel may have closely relationship in the signal occurrenceand transmission. To study their mechanisms of gating and inactivation of ion channels，isa great benefit to explore the relationships between the structure and function of the ionchannels and discover the molecular mechanisms of some diseases and drug targets.In this study，with the help of several techniques such as molecular dynamicsmodeling，cell culture，immunofluorescence，electrophysiology，we studied the couplingeffect between the CaV1.2α1（a homolog of L-VGCCsα subunit）and mslo（a homologof BK channel α subunit）. The main conclusions comprise as follows：CaV1.2α1andmslo can partly colocation on celluar membrane when they coexpressed in HEK293cell，and CaV1.2α1channels are voltage-acticated. Inward current of CaV1.2α1leads to therising of local Calcium ion concentration,indicating that it has partly coupling effectwith BK channel on structure and fuction.