Biosensor Based On DNA Probes For The Detection Of P53DNA And Coralyne

DNA probes have been developed rapidly with improvement of modernbiotechnology, development of solid-phase chemistry and invention of automatedDNA synthesizer. Because of their specific recognition for a variety of targets andflexible signal conversion mechanism, a series of ingenious designs of new DNAprobe based biosensors have been developed for geneanalysis, disease diagnosis,health and epidemic prevention, drug mechanism analysis, bio-engineering, etc. Inthis thesis, DNA probe based biosensors have been developed for simple, cheap andsensitive detection of p53DNA and small molecule drug.1. Using micro-pore DNA biosensor and commercially available portable glucosemeters for p53DNA detectionBecause of their low cost, simple operation and reliable quantitative results,portable glucose meters have been used to integrate with micro-pore DNA sensor forstructuring a biosensor for p53DNA. This simple biosensor can detecte a seriesconcentrations of p53DNA with highly sensitive simultaneously. Most important, themethod converts the p53DNA detection to glucose detection, which only need acommercial and portable glucose meter. Finally, the limit of detection is as low as1pM, and the biosensor is specific, which even can distinguish mismatched DNA.2. Using micro-channel DNA biosensor and commercially available portableglucose meters for p53DNA detectionSince glass has the advantages of transparency, corrosion resistance, wettingability and capillary function and so on, then we design a micro-channel type chipwith a glass-gold film which is easy to modify. In order to simplify the productionprocess and reduce the sensor cost, we replace the expensive gold film with the cheapglass, and then designed glass-glass micro-channel type chip. In addition, integrationwith portable glucose meter, a new biosensor has been developed with the similardetection principle mentioned in the chapter above. the method convert the p53DNAdetection to sucrose detection, realizing sensitive detection of p53DNA. The sensor iseasy to fabricate with simple operation, and it greatly reduced the cost of testing, andit is expected to be used in production on a large scale with wide application inmedical and others.3. High sensitive coralyne detection by using of Au nanoparticles-enhanced surface plasmon resonance (SPR) biosensorAn Au nanoparticles-enhanced SPR biosensor for high sensitive detection ofadenosine (A) was developed. It was based on the specific coordination betweencoralyne and A with a stoichiometry of one coralyne per four A. The A-rich DNAstrands modified on Au nanoparticles and on Au film respectively could form theduplex. SPR signal was enhanced by the electronic coupling between the localizedplasmon of Au nanoparticles and the surface plasmon wave associated with Au film.The results showed that the SPR sensor was highly sensitive, and the limit ofdetection was ca.0.07pmol/L. It was about5-6orders of magnitude lower than thoseof fluorescent and colorimetric methods.