Cloning And Characterization Of Growth Hormone Gene From Vdricorhinus Macrolepis

The Varicorhinus Macrolepis, a kind of precious and wild small fish in Mountain Tai,was one of those endangered species. It has high nutritional and special remedy value, butvery demanding requirements to its living environment and growth is extremely slow.To study growth and development of Varicorhinus Macrolepis, we amplified the cDNAof growth hormone (GH) gene from it with SMART RACE technology, and analysis thestructure and the functional sites of nucleotide and amino acid sequence using a few ofbioinformational tools. It is to establish the foundation for the further protection andexploiture of Varicorhinus Macrolepis. The results are shown in detail as follows:(1) We have cloned the full-length cDNA of GH of Varicorhinus Macrolepis.(2) The full-length of cDNA sequence of growth hormone (GH) gene from VaricorhinusMacrolepis is1307bp (Genbank ID: JN565026), including5’UTR of167-bp,3’UTR of552-bp, and an ORF of588-bp, that encoded a polypeptide of195amino acid residues. Thephylogeny tree analysis revealed that Varicorhinus Macrolepis had a relative high homologywith Carassius auratus and Cyprinus carpio, suggesting that it has close genetic relationshipwith Carassius auratus and Cyprinus carpio. No apparent differences in categories andnumbers of function site of GH protein were observed between Varicorhinus Macrolepis andother species in Cyprinus family. Then, we found that5conserved domains which are existedgenerally in Growth hormone protein. The second and tertiary structure of the GH protein wasanalysised, which contains typical four alpha helixes with reversed parallel.Collectively, we firstly reported growth hormone (GH) gene from VaricorhinusMacrolepis in Mountain Tai, our findings provide a new line of evidence for research ongrowth and development of small fish in Mount Tai.