The Study Of Estrogen Regulated MicroRNA Profile In Mouse

Since chemicals that have estrogen-like activity in environmental Endocrine pollutants in1990s, these chemical have caused high attention in environment research and political aspects. These chemicals have similar function with natural estrogen, they can regulate endocrine system development and change Physiological function, and have strong effective in low concentration. These substances are thought to cause adverse effects on development or function of reproductive organs by binding to estrogen receptor as estrogen mimics. Especially, exposure to these chemical during developing stage may cause dysfunctions or abnormalities of reproductive organs later at adult stage, which may lead to infertility or development of cancer (prostate cancer, breast cancer,).With development of industry, categories and numbers of these chemical with estrogen activity are more and more huge in environment. It is necessary to look for a method, that can quickly and comprehensive detect environmental estrogen.Toxicogenomics method was used in this study, and made a originality is that replace mRNA Geneship by miRNA Geneship. We chose estradiol as Standard estrogen and chose mature female mouse without ovary and mature male mouse and immature female mouse as three animal models in order to found miRNA expression change profile after administration estrogen, respectively.We found that in this study:(1)uterine weight exhibited increase after estrogen treatment in mature and immature female mouse model (2) There are31miRNAs have been proved have significiane change in mature male mouse model. The results determined mature male mouse may be a good model used to check estrogenic compounds.(3)11miRNAs were screened out for significant fold change in two types Genchip. Real-time PCR was performed to further confirm that4of7selected miRNAs, miR-451, miR-155, miR-335-5p, and miR-365, are E2regulated miRNAs in the uterus. The predicted targets of these miRNAs have very focus function of cell grow control, which is consistent with the main E2function. MiR-451had similar strong responses to E2in the uterus of both immature and overiectomized mice, and could be a potential biomarker for estrogenicity in the uterus.