The Protective Effect Of 7,8-DHF On PC12 Cells Injury-Induced By 6-ohda

The present study aims at investigating the protective effects of 7,8-DHF against the toxicity of 6-OHDA and the molecular mechanism.In this research,we used 6-OHDA to injure PC 12 cells.MTT was used to observe the protective effects of 7,8-DHF against 6-OHDA and the blocking effects of tyrosine TrKB antagonist ANA-12,K252a and PI3K antagonist LY294002.Moreover, Hoechst 33258 staining,Reverse translation-PCR, flow cytometry and enzyme linked immunosorbent assay were used to observe the apoptosis of PC12 cells,the expression of TrKB and the self-anti-oxidative stability.Results are as follows:1. 6-OHDA decreased cell viability of PC12 in a dose-dependent manner(P<0.001). 7,8-DHF attenuated the toxicity of 6-OHDA(P< 0.001).The maximal rescue occurred at the concentration of 25μM.2. The neuroprotective effect of 7,8-DHF on cell viability against 6-OHDA-induced toxicity was completely blocked by LY294002 (P<0.001)3. 6-OHDA can significantly induce the apoptosis of PC 12 cells (P< 0.001),7,8-DHF attenuated the apoptostic rate of PC12 cells induced by 6-OHDA(P<0.001).4. 6-OHDA significantly decreased mitochondrial membrance potential in PC12 cells(P <0.01).7,8-DHF could significantly reverse the toxic effects of 6-OHDA (P< 0.01)5. 6-OHDA significantly caused the increase of Malondialdehyde (MDA) (P< 0.05).Treatment with 7,8-DHF preliminarily increased the quantity of MDA controlled with the 6-OHDA group(P<0.05).6. Treatment with 7,8-DHF preliminarily can significantly cause the increase of Superoxide Dismutase(SOD) activity (P<0.01)These data demonstrated that 7,8-DHF have neuroprotective effects against the 6-OHDA-induced neurotoxicity in PC 12 cells and their actions might involve inhibiting PC12 cells apoptosis,activation of PI3K/Akt signaling pathways and the self-anti-oxidative stability of 7,8-DHF.These results not only provide new mechanism for the neuroprotective effects of 7,8-DHF,but also provide experimental evidence for the clinical application of 7,8-DHF for prevention and treatment of Parkinson’disease.