The Bioinformatic Reaserch Of PIGR And MYB Gene Family

Immunoglobulin A is the primary protective barrier of human and animal digestivetract, respiratory and genitourinary mucosal surface and is the first line that defenses theenvironmental pathogens and antigens. The formation of Secretory immunoglobulindepends on the epithelial cell surface poly-immunoglobulin receptor endocytosis. pIgAinteract with the PIGR on the basement membrane of epithelial cell. Cell endocytosis andtranscript it to the apical membrane. Then, the extracellular ligand-binding segment ofPIGR is hydrolysised and the segment to gether with the pIgA is released to the mucus. SCdose not have only non-specific antibacterial effect, but also can prevent the pIgA fromdegradation. PIGR not only plays a key role in transferring the pIgA in the epithelial cells,but also is a key factor in mucosal immunity.MYB gene family is an important transcription factor family, is widely distributed inanimals, plants, fungi and protists. Each MYB domain conserved 51 or 52 amino acids, canform a helix - helix - turn–helix structure, the last two helix of which play an importantrole in DNA binding. MYB is divided into four categories 1R-MYB/MYB-related,R2R3-MYB, R1R2R3-MYB, 4R- MYB , depending on the number of adjacent repeats. Inplants, R2R3-of MYB is one of the largest transcription factors involved in the networkregulation of plant growth and metabolite , response to biological and abiotic stress, thesymmetry development of flower and other life processes.In this paper, we use bioinformatics methods to compare PIGR. The comparativeanalysis of the whole sequence and tertiary structure prediction proved that the evolutionof the PIGR maintain consistency with the evolution of species. Various functional areas ofPIGR were compared,and we found that the evolution of the IG-like domain have othermechanisms of formation. Our results show that higher organisms IG-like domain, D3/D4and D4/D5 ,may derived from a bony fish D1, D2; D1, D2, and D3 may be from D2 of thebony fish ,During the comparative of Hydrolysis sites, sorting signals, endocytosis signalsand transfer of signal comparison in teleosts, amphibians, birds, mammals, we found thatthe teleosts have less corresponding loci, which implies that PIGR play its uniqueadjustment mechanism in teleosts.We analysis the MYB gene family by bioinformatics. We gain the members of theMYB gene family in four species (192 genes in Arabidopsis, 514 genes in soybean, 173 genes in Medicago truncatula, 183 genes in Lotus corniculatus), and rename the MYBgene family of soybean. In four species, we have various species-specific MYB gene, somegenes duplicate in the evolutionary process, and some genes are lost. There are largechanges in the MYB gene family in gene structure, intron number from 0 to 22, and thedistribution of genes concentrate in several chromosomes ; the previously report shows thatinterval region between two MYB domain is 5 or 6 amino acids.we find that the intervalregion has about a domain size in four species. These differences may be associated withtheir functions. In the comparison process, we get some of the genes which differentspecies have , these genes may be used to explain the mechanism of some speciesphenotypic differences.The PIGR and the MYB gene family have played an important role in differentspecies.The analysis of PIGR helps us understand the mucosal immunity, and providesome basis for the further analysis of the teleosts PIGR. The analysis of the MYB genefamily provide some ways to study gene family in different species, and provide a favor tofurther analysis the function of the MYB gene family.