Seasonal Variation Of Arbuscular Mycorrhizal Fungi In Endangered Plants Circaeaster Agrestis And Sinopodophyllum Hexandrum Roots

Arbuscular mycorrhizal fungi (AMF) are widespread in nature environment and can form symbiont with many plants. The diversity and changes of AMF determine the vitality of plants growing. This paper uses the traditional Morphological identification, combine with building gene library and PCR-DGGE and other molecular biology technics to have a seasonal AMF diversity research on the endangered plant Circaeaster agrestis and endangered medicinal plant sinopodophyllum hexandrum in Xinglongshan region, Gansu. We have reached these conclusions via the research result data analysis:1. Spores density in Circaeaster agrestis environment soil different from each season. In May the spores density reaches its peak, and in July the spores density is minimum. There is no notable change between the spores density in May and in October, but there is a difference to the spores density in July.2. There is a seasonal species variation of the spores in Circaeaster agrestis environment soil, the Glomus and Acaulospora are the dominant genera. Glomus keeps inclining from seasonal change (May, July, October), and the Acaulospora keeps declining through the season change.3.The AMF colonization in Circaeaster agrestis roots has a seasonal variation, the arbuscular colonization (AC) declines from spring to autumn.4. The spores density change is correlated with the organic carbon, available phosphorus and pH in Circaeaster agrestis environment soil; the AMF species seasonal diversity in Circaeaster agrestis roots is correlated with the organic carbon, available phosphorus, available nitrogen and pH in Circaeaster agrestis environment soil.5. Spores density in sinopodophyllum hexandrum environment soil different from each season. In March the spores density reaches its peak, and in July the spores density is minimum. There is no notable change between the spores density in March and in October, but there is a difference to the spores density in July.6. There is a seasonal species variation of the spores in sinopodophyllum hexandrum environment soil, the Glomus is the dominant genera.7. The root length colonization and arbuscular colonization of AMF in sinopodophyllum hexandrum roots in March is lower than in July and October, and the vesicular colonization in March is higher than the vesicular colonization in July and October.8. There is a seasonal species diversity of AMF in sinopodophyllum hexandrum roots, the species diversity trend is July>March>October. Glomus is the dominant genera, Scutellospora only existed in July and did not appear in the samples in March.9. There are4genera11species identified from the Circaeaster agrestis environment soil, they are Scutellospora erythropa, Acaulospora rugosa, Glomus mosseae, Glomus etunicatum, Acaulospora colossica, Glomus. Sinuosum, Glomus tortuosum, Acaulospora delicata, Gigaspora decipiens, Glomus callosum, Glomus eburneum.10. There are6genera15species identified from the sinopodophyllum hexandrum environment soil, they are Gl. Caledonium, Gl. Constrictum, Gl. Diaphanum, Gl. Eburneum, Gl. Etunicatum, Gl. Sinuosum, Gl. Minutum, A. denticulate, A.polonica, A. spinosa, Sc.coralloidea, Sc. Pellucida, Pacispora franciscana, E. baltica, Paraglomus occultum.