The Research Of Gene Interaction Network Based On Endogenous SiRNA

RNA interference (RNA interference, RNAi) is the gene silencing phenomenon after transcription that induced by double-strand small interfering RNA (small interfering RNA, siRNA), can degrade the target mRNA or inhibit the translation. It is also one of the phenomenon that is ancient and highly evolution conservative of biosphere. This article aims to find an evolution conservative gene interaction networks based on RNAi induced by the endogenous siRNA, by using the principles and methods of bioinformatics. We obtained the genome datasets of multiple species, respectively predicted the siRNA in intron and its target genes of each species genome. Further we respectively counted the distribution of these siRNA and their target genes on the molecular function, cellular components and biological processes.Through the comparison of the statistical results, it shows that the endogenous siRNA in the part of molecular function, the activity of enzyme is significantly the highest, in the part of cell components, the cellular components is significantly the highest, in the part of biological processes, they involve to regular multiple physiological processes. This shows that siRNA genes and their targets play an important role in various life stages of individual, which play a regulation role at the beginning of individual development. According to this conclusion, we predict that there is a conservative gene interaction networks which is induced by siRNA in biological individuals. Thus, we choose C. elegans as a research object for validation.