The Induction Of Honeybee Simple Flight Behavior And Optic Flow Stimulus-spike Potentials Relationships

Insect robot is a novel cyborg, formed by insect itself and control module. With the combination of the advantage of the flight performance of insects and the control ability of micro-electronic modules, it will have wide applications in scientific research, defense, security and media fields. The study of behavior-related neuromuscular system and the relationship between stimulation and induced behavior are the basis for insect robots to control the specific behavior of insect accurately. This thesis focuses on the responses of honeybee stimulated by electrical pulses and optical flow. Experiments are conducted to collect the neural electrical signals for further analysis of flight performance.①Electrical stimulation is the most successful method for biological robots. This thesis try to stimulate optic lobe, antennal lobe, the dorsal longitudinal muscle and the dorsal ventral muscle of tethered/half-tethered honeybee by alternating positive and negative pulses. The experimental results show flight initiation and cessation can be reproducibly generated using electrical pulses between two electrodes implanted into the optic lobes. The parameters of pulses are 4V-40Hz-5ms-30 (pulse amplitude-frequency-width-number) and the success rate flight initiation and cessation are more than 60% and 90%. The mean response time from stimulation to flight initiation for ten honeybees is 0.885 s and mean flight duration is 9.35 s. The effect of different stimulus patterns on the honeybee's behavior and parameters including success rate, response time and flight duration are compared and modeled. The results enable us to choose the most appropriate stimulus parameter (4.8V-55Hz-1.2ms-25) and determine the stimulus protocol (optic lobe).②Asymmetric optic flow stimuli designed by LED array are offered to stimulate the left and right compound eyes. Then the spike signals of the optic lobes are recorded and further analyzed for filtering out the interference signal and calculating the number of spikes in the unit time. The results show that the firing rate of the optic lobe neurons under the table lamp irradiation is larger than normal light. When the optic flow stimuli of the left eye are more strongly than the right, the firing rate of the left optic lobe neurons are higher than the right accordingly.