Study On The Immune Regulation Function Of5-HT Receptor

5-hydroxytryptamine (5-HT) also known as serotonin, is a monoamineneuromodulator.In recent years, studies have found the5-HT and its receptors also exists inimmune system,5-HT as an immunomodulatory factor perspective has been graduallyaccepted by people. To explore the issues of5-HT receptors on immune regulation function,research contents include:1. We studied the effect of5-HT1A receptor agonists naftopidil and5-HT2A receptorantagonists quetiapine fumarate on boiler antibody titer afer inoculated Newcastle diseasevaccine by hemagglutination inhibition assay.The experimental result showed:Comparing tothe control group of21day old chickens, antibody titer of5-HT1A receptor agonist in midand low dose group (0.42mg every chick/day,0.21mg every chick/day) was lower than that inthe control group, high dose group (0.84mg every chick/day) was higher than that in controlgroup, while the5-HT receptor antagonist groups (1.64mg every chick/day,0.82mg everychick/day,0.41mg every chick/day) were higher than those in the control group, all theresults were not significant.2.We studied the effect of5-HT1AR,5-HT2AR agonists and antagonists on ovineperipheral blood T lymphocyte transformation in vitro by MTT assay. The experimental resultshowed: Within10μM to100μM concentrations,5-HT1A receptor antagonist and5-HT2Areceptor agonists dose dependent inhibit ConA stimulated T lymphocyte proliferation, whilesignificantly promote lymphocyte proliferation activity in resting state.The result indicate that5-HT1A and5-HT2A receptors are involved in the opposite regulation of lymphocyteproliferation.3. We also detected the effect of5-HT1AR ASODN and5-HT2AR ASODN on ovineperipheral blood lymphocyte proliferation in vitro by MTT assay. The experimental resultshowed:0.4μM5-HT1AR ASODN significantly inhibit ConA stimulated T lymphocyteproliferation,0.4μ M5-HT2AR ASODN significantly promote ConA stimulated Tlymphocyte proliferation,0.4μM5-HT1AR,5-HT2AR SODN both significantly promoteConA stimulated T lymphocyte proliferation.While,5-HT1AR and5-HT2AR ASODN has significantly increased lymphocyte proliferation activity in resting state.4.We detected the effect of5-HT1AR,5-HT2AR agonists and antagonists on IL-2,IL-4concentration of culture liquid of ovine peripheral blood lymphocyte. The experimental resultshowed: Different dose of5-HT1AR,5-HT2AR agonists and antagonists can significantlyincrease or decrease IL-2, IL-4concentrations of lymphocyte culture liquid, but there is nodose dependent effect.These chief conclusions below are yielded from this experimental study:1.5-HT receptor may be involved in the regulation of humoral immune in chicks.2.5-HT1AR promote ConA stimulated lymphocyte proliferation,5-HT2AR inhibitConA stimulated lymphocyte proliferation.3. Inhibition of5-HT1AR or activation of5-HT2AR can enhance proliferation activatyof resting lymphocyte.4.5-HT1AR,5-HT2AR may be involved in the regulation of the secretion of IL-2andIL-4by lymphocytes.