| 5-HT (5-hydroxytryptamine), also known as serum angiotensin, is a chemical of indole amines. A lotof literatures suggest that5-HT plays a very important role during the recovery period after the liverresection in rats.We found that the number of5-HT secretion cells in gastrointestinal tract increase greatlyafter PH,and the distribution of5-HT in liver and blood changes too. How the hell does PH effect thesecretion of5-HT?And what have PH done to gastrointestinal tract?Unfortunately, there is only a little sumof papers taking statements on it.To know the relationship of PH and5-HT secretion, this paper focuses onwhat changes of the structure and function and the number of bacterial of gastrointestinal tract,and thecontact between them.In this paper,we used rats as experimental animals. Using transmission electron microscopy toobserve the impact of PH on the ultrastructure of rat intestinal mucosa, making use of the methylene bluedye as an indicator to detect the change of gastrointestinal motility in rat after liver resection. Using thecolony culture and plate counting method for detecting the number change of intestinal bacterial in rat afterPH and through the injecton of gentamicin sulfate(a kind of antibiotic) in rats after PH as an aided analysis.Using high performance liquid chromatography(HPLC) Ultraviolet Spectrophotometry analysis thechanges5-HT secretion in rat blood in different periods after PH and through the injecton of gentamicinsulfate(a kind of antibiotic) in rats after PH as an aided analysis.The main results as follows:(1) the ratileal and colonic mucosa microvilli show up unequal heights and arranged loosely scattered during the timeof1.5-24h after PH;the gap among some epithelial cells of mucosal appeared broadly, and the boundary ofcell is not clear;the mucosal endocrine cells were in active state,the secretory granules increased and filledup in cytoplasm, the cytoplasmic processes were significantly being,there were also visible small vacuolesin the protruding parts because of discharged of secretory granules; bacterial adhesion were common on theinner surface of the ileum and colon wall.(2) The moving distance of methylene blue staining in ratgastrointestinal were significantly smaller than that of the control group during2hours after PH, P＜0.01；in normal rats methylene blue staining can go through the small intestine completely in2hours withgastrointestinal peristalsis,but it required at least5h for the rats in PH group to go completely through the small intestine.(3) In0h,2h,6h,12h,24h,48h and72h after PH, the quantity of bacteria in rat intestinalincreased significantly in each period compared with the control group,P＜0.01；the number of intestinalbacteria of rats of the PH and administrated antibiotic groups decrease significant compared with PHgroup,P＜0.05。(4) In0h,2h,6h,12h,24h,48h and72h after PH,the content of5-HT in rat blood increasedsignificantly in each period compared with the control group,P＜0.01；the5-HT conten in rat blood inorally gentamicin sulfate group reduced significantly compared with PH group,P＜0.05。Based on theabove results, we drawn the conclusions as follows:(1) rats' intestinal absorption and barrier function isweakened during1.5-24h after PH,but its secretory function enhanced;(2) the rats' gastrointestinalperistaltic function decline in short-term after PH;(3) the number of bacteria in rat colon and ileumincrease significant in12h after PH,and became stabilizing in48h after PH, orally gentamicin sulfaterestrain bacteria greatly;(4) as a condition of stress, PH effects greatly on the intestinal barrier,gastrointestinal motility, intestinal bacteria and so on In the short term,which lead to the secretion of5-HTin the blood of rats increased. |
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