PTSD After The Disaster Rescue Survey And Serological,5-HTTLPR Gene Polymorphism

In recent years, natural disasters, man-made disasters occur frequently, post-traumatic stress disorder (Post-Traumatic Stress Disorder, PTSD), significantly higher rates of PTSD is the sudden, threatening or catastrophic life events lead to the individual delay the emergence and long-term persistence of mental disorders and its clinical manifestations in order to re-experience the trauma characteristics, accompanied by a mood of irritability and avoidance behavior has serious social and family dangers of war, social violence, major accidents and natural disasters and other trauma in the accident, PTSD has a high incidence of recent years has become the new hot spot in the psychiatric, psychological studies, however, the domestic in this area less systematic research.Not only the occurrence of PTSD in victims also occurred in the scene witnesses and rescue workers, armed police forces to shoulder the domestic and international relief, the mission of the National Counter-Terrorism at the sudden and respond to emergencies in the implementation of the tasks in the face of the complex scene of the disaster area or at the sudden and tragic trauma of the accident, can easily lead of PTSD.In this study, the armed police forces involved in the earthquake and other disaster relief, one year after the rescue, through the PTSD questionnaire screening for detection of serological markers and the detection of5-HTTLPR gene in order to explore the influencing factors of the occurrence of PTSD, the possible pathogenesis The relationship between and5-HTTLPR gene polymorphism.Part1The first part of post-traumatic stress disorder questionnaireObjective:In this study, police officers and soldiers of the Armed Police Force to participate in earthquake rescue mission through the relevant questionnaire for PTSD screening, to study the impact of PTSD-related factors and PTSD patients screened for psychological intervention in order to improve the armed police forces carry out the ability of non-combat operations.Methods:1Selection of subjects:Yushu earthquake rescue mission in2010one year after the disaster relief, select the Armed Police Force780officers and men with PTSD screening scale (the post-traumatic stress. Disorder checklist. Civilian version, PCLC) conducted a questionnaire survey of selected officers and men of the SCL-90self-rating scale. Usually carry out the tasks of counter-terrorism at the sudden the officers and men of the Secret Squadron305, carry out usual daily service tasks to the officers and men395, logistical support personnel (Chuishi Ban officers and men)80.2PCL-C and the SCL-90questionnaire:1year after the Yushu earthquake relief selected officers and men face to face questionnaire survey, structured interviews suspicious PTSD patients by the professionalism psychiatrist, in line with the spirit of America diagnostic and statistical Manual4th edition of PTSD diagnostic criteria for the diagnosis of PTSD symptom self-assessment and the SCL-90scale.Results:1By the psychological survey questionnaire screening invalid questionnaires,780questionnaires184were removed, the remaining596valid questionnaires, usually carry out the mandate of the Unexpected Terrorism officers and men of the Secret Squadron235valid questionnaires, usually carry out the task of officers and men of the day-to-day service to283people,78support staff, and effective questionnaires confirmed18patients with PTSD, PTSD prevalence rate of3.02%.2Of PTSD incidence of participating in the rescue mission of the Armed Police Force police, family background, as rescue task differences have a significant correlation of PTSD group and non-PTSD group difference was statistically significant (p<0.05).3The SCL-90scale, all survey psychological survey and found to rescue the officers and men in the somatization, obsessive-compulsive symptoms, interpersonal sensitivity, depression, anxiety, compared with the norm in the difference was statistically significant (p<0.05) hostility, phobia, paranoia, mental symptoms, compared with the norm, the difference was not statistically significant (p>0.05).Conclusion:1the officers and men to participate in the task of earthquake rescue, suffering from PTSD, one year later there are still3percent of the officers and soldiers suffering from PTSD, a more serious impact of a major disaster on rescue personnel.2Of PTSD have a significant correlation with the police Armed Police Force participated in the rescue mission, family background, as rescue tasks different.3Measured from the SCL-90showed that the officers and men of the rescue after the earthquake in the body, obsessive-compulsive symptoms, interpersonal sensitivity, depression, anxiety, there have been notable exception.Part2Some cytokines in patients with PTSD serum and related metabolitesObjective:Study the expression of cytokines and metabolites of the stress response, and explore the development of PTSD mechanism is expected to provide valuable information about the intervention of PTSD and provide the basis for the exploration of new clinical interventions. To this end, we further part of the cytokine and related metabolites in patients with PTSD to explore oxidative stress, inflammation in the possible role of PTSD onset.Method:(PTSD group) of18cases and32cases did not participate in the relief of the same unit of healthy volunteers (control group), serum MDA, the ACTH and IL-6, NE NO, IL-8concentration of HSP72and total plasma SOD activity level, Determination of the human vasoactive intestinal peptide (VIP). Age groups18-25years, male, a normal diet, eliminate history of neurological or mental illness, no wine or a history of drug abuse. There was no significant difference between the two groups in age, sex, and complicating diseases.Result:Found that PTSD patients of MDA in serum, ACTH and IL-6, NE, NO and plasma SOD activity level determination of significant differences between the PTSD group and the non-PTSD group (P<0.05). No difference between IL-8, of HSP72, human vasoactive intestinal peptide (VIP).Conclusion:PTSD patients serum MDA, NE, NO, IL-6concentration compared with the control group increased. SOD activity and ACTH levels were decreased. Oxidative stress, inflammation, and adrenergic system in the incidence of PTSD plays an important role.Part3Correlation analysis of the5-HTTLPR gene polymorphism and post-traumatic stress disorderObjective:In this study, polymerase chain reaction (PCR) technology, a group of patients with PTSD5-HTT gene promoter region (5-HTTLPR) insertion/deletion polymorphism studies to explore the5-HTTLPR polymorphism and trauma The relationship between stress disorder (PTSD).Methods:The use of PCR technology, case-control study of53cases of PTSD patients (18cases of PTSD patients based survey,35cases diagnosed three months after the disaster relief in patients with PTSD) and50cases of normal control group polymorphism AnalysisResults:And detected two fragments, of484bp (S),528bp (L) of PTSD group allele frequencies with the normal group the difference between statistical significance (P<0.05), of PTSD group S/L genotype The frequency is higher than the normal control group.Conclusion:That in this group of Chinese population,5-HTTLPR gene polymorphism associated with PTSD, including S-type is often seen in PTSD patient.