Correlation Of The Genotype And Mutation Of HBV As Well As X Protein In HCC

Objective:To comprehend the correlation of HBV genotype,1762/1764and1896mutation as well as X protein in HCC for further investigation about the pathogenesis and theoretical basis about prevention, cure and early diagnosis of HCC.Methods:HBV genotype and mutation of80samples including normal, chronic HBV carrier, chronic Hepatitis B, hepatitis B Induced cirrhosis and HBV-associated HCC were tested by NAAT and sequencing method, sequenced by the auto genotype analyzer and tested by the quantitative method and chromas program. The quantization of HBV-DNA was detected by FQ-PCR. Expression of X protein in above-mentioned5groups liver tissues was detected by immunohistochemistry staining mesthod and results were judged by Semi-quantitative integration. Used ELISA tested the HDVM of the HBV serums antigen antibody reaction and analyzed the result by statistical methods. All the testing data were processed statistically by SPSS17.0statistical software package.Results:1,Among the78chronic HBV infectors, there were31cases genotype B with the proportions39.7%(31/78), while47cases C with60.3%(47/78). There were no genotype A,D,E,F,G and H found in this research. Genotype C is the main HBV genotype in Yunan province. The proportion of Genotype C in group ASC, CHB, LC and HCC was42.9%(6/14),50.0%(15/30),64.3%(9/14) and85.0%(17/20), and the discrepancy had statistical significance(χ2=8.29, p=0.04). While the proportion of genotype C was overwhelming in group LC and HCC, but the discrepancy of distribution in the two groups had no statistical significance(p=0.161). Combining of the two groups(26/34) was compared with group CHB and ASC(21/44), and the discrepancy had statistical significance(x2=6.62, p=0.01). The proportion of genotype C in group LC and HCC was higher than in group CHB and ASC.2,There were50cases with BCP mutation and44PC mutation in78chronic HBV infectors. The proportion of BCP mutation with genotype C in HBV infectors was21.4%(3/14),43.3%(13/30),50.0%(7/14) and80.0%(16/20), while PC mutation was14.3%(2/14),40.0%(12/30),57.1%(8/14) and75.0%(15/20), both of the two mutation were more than HBV infectors with genotype B, and the discrepancy had statistical significance (p<0.05) except group ASC.3,Expression of X protein in group LC was stongest with positive rate71.4%, next came group HCC (70.0%), CHB (60.0%) and ASC (50.0%) and the discrepancy among groups had statistical significance(P <0.05). Combining of group LC and HCC(70.6%) was compared with group CHB and ASC(56.8%), and the discrepancy of X protein expression in the two had statistical significance(P<0.05). Strong positive cases of X protein expression in group LC and HCC were more than group CHB and ASC4,Positive rate of X protein expression in chronic HBV infectors with genotype C in group ASC, CHB and HCC was35.7%(5/14),40.0%(12/30),70.0%(14/20), which was higher than in genotype B, that was14.3%(2/14),20.0%(6/30)and0%(0/20). The discrepancy had statistical significance(P <0.05). The proportion of HBV infectors with positive BCP mutation and positive X protein expression in the four groups was71.4%,72.2%,70.0%and85.7%, while proportion of positive PC mutation was57.1%.66.7%,80.0%and57.1%, both were higher than HBV infectors with negative BCP and PC mutation. Conclusion:1,There are genotype B and C, mainly genotype C existing in chronic HBV infesters (including chronic HBV carriers, chronic Hepatitis B, Hepatitis B Induced Cirrhosis and HBV-associated HCC) in Yunnan Province, and there were no other genotypes found. There is a connection between the genotype of chronic HBV infectors and the clinical spectrum of disease, genotype C has connection with the relatively serious liver disease(Hepatitis B Induced Cirrhosis and HBV associated with HCC).2,There were more BCP and PC mutation with genotype C than B in every group.3,Expression of X protein in group LC was stongest, next came group HCC, CHB and ASC. Strong positive cases of X protein expression in group LC and HCC were more than group CHB and ASC.4,Positive rate of X protein expression in chronic HBV infectors with genotype C was higher than genotype B. There was obvious BCP and PC mutation in chronic HBV infectors with positive X protein expression. Correlation maybe exists among X protein expression, genotype C as well as BCP and PC mutation.5,HBV genotype C, BCP and PC mutation as well as X protein are related with the occurrence and development of HCC. But whether there is cooperation among them, whether they are related with disease development, and the definite mechanisms that they advance the ocuurence of HCC are still need further research by extending the numbers of cases and expanding research area.