The Effect Of MiR-183Expression In Colorectal Cancer

Objective:quantitative RT-PCR was used to detect the expression of miRNAs in colorectal cancer tissues and adjacent normal colorectal tissues, in order to investigate the expression of miRNAs which are tightly related to the development of colorectal cancer. To predict the targets of these miRNAs with bioinformatics and knowledge of colorectal cancer. Finally, preliminary verification of the predicting results.Methods1. looking for the miRNAs which have close ties to colorectal cancer by reading and investigating literatures. The expression profiles of miR-183were compared between colorectal cancer tissues and adjacent normal colorectal tissues using quantitative RT-PCR method.2. Using bioinformatics to predict the targets of miR-183.3. PDCD4were detected at their protein levels by immunohistochemical (IHC) staining between colorectal cancer tissues and adjacent normal colorectal tissues. Then, the functional effect of miR-183in colorectal cancer was preliminary examinedRusults:1. we found some over expression levels of miRNAs in colorectal cancer by document retrieval. such as the cluster of miR-181and miR-183.2. Among the10colorectal cancer samples analyzed, microRNA-183was significantly up-regulated in8samples compared with the matching nontumoral colorectal tissues.3. Up-regulation of miR-183expression was correlated with lymphnode metastasis in colorectal cancer.4. In colorectal cancer tissues, the expression of PDCD4protein was obviously decreased campared with the matching nontumoral colorectal tissues.Conclusions:We preliminary put forward that miR-183may play an important role in colorectal cancer development by repressing the expression of PDCD4.