| Objective through the establishment of rabbit extra-articular tendon-bone healing model,respectively, apply histological and biomechanical for the experimental specimens to explorethe Effect of autologous bone marrow mesenchymal stem cells on allogeneic bone andautogenous tendon healing.Method Adopt New Zealand white rabbit bone marrow by bone marrow biopsy methodand use density gradient centrifugation to isolate and culture in vitro amplification andidentify of bone marrow mesenchymal stem cells.47healthy adult New Zealand whiterabbits were sacrificed2as a source of allogeneic bone.45New Zealand white rabbits wererandomly divided into three groups: first group, autologous tendons and composite bonemarrow stem cells in allogeneic bone of tendon-bone fixation group. The second group,Autologous tendons and not compound bone marrow mesenchymal stem cells in allogeneicbone of tendon-bone fixation group. The third group, autologous tendon and autologousbone of tendon-bone fixation group. Model: the inside of the tibial tubercle established about5mm in diameter bone hole to take the patellar ligament as a fulcrum to the tibial tuberositymedial1/2flip implantation of bone inside the cave. first group, composite bone marrowmesenchymal stem cells in allogeneic bone filling the bone hole wrapped tendon.the secondgroup, with not compound bone marrow mesenchymal stem cells in allogeneic bone fillingthe bone hole wrapped tendon. The third group, with autogenous bone filling the bone holewrapped tendon. Postoperative respectively feeding cage. At3,6, and12weeks, the threetime points for histological examination, each of the three rabbits. Taken biomechanicaltesting at6and12weeks of the two time points, each of the three rabbits. SPSS17.0statisticalsoftware, the data collected were analyzed by using Single factor analysis of variance and SNK-qinspection, inspection level a=0.05, p <0.05statistically significant.Result histological observation: postoperative three weeks, the first group: fibroblastproliferation in the tendon bone interface is active and showing the collagen fibers.largeamounts of inflammatory cells were saw. The second group: Between the tendon boneinterface exist active proliferation of fibroblasts and a large number of inflammatory cells,collagen fibril is little. The third group: the fibroblasts in the tendon-bone interface is activeand large amounts of inflammatory cells are existde, showing that collagen fibril formationand active osteoblast proliferation. postoperative six weeks, the first group: Between the tendon bone interface fibroblasts become fiber cells, showing ordered collagen fibers. Thesecond group: fibroblasts and collagen fibers are seen between the tendon bone interface,Collagen fibers and new bone is loose. The third group: Seen a large number of collagenfibers arranged in order to form linking closely with the new bone. postoperative twelveweeks, the first group, the tendon-bone interface is similar to the normal fibrocartilage of thetendon bone connection. The second group:: a small amount of collagen fibers and bone arefusion. The third group: the tendon bone interface is sharpey fiber formation.Biomechanical test results: the first six weeks of biomechanical test results showedthat: the first group and third group of the pullout load was significantly better than the secondgroup. The first group and third group is not significant difference. The first12weeks ofbiomechanical test results showed that: the maximum pull out load of the three groups is notsignificant difference, do not have statistically significant.Conclusions (1) in the early of tendon-bone healing compound of BMSCs with allogeneicbone and autologous tendon of tendon-bone healing in histology and biomechanics is superiorto not compound BMSCs with allogeneic bone and autogenous tendon of tendon-bonehealing.(2) in the early of tendon-bone healing autologous bone and autologous tendon oftendon-bone healing in histology and biomechanical is superior to not compound BMSCswith allogeneic bone and autogenous tendon of tendon-bone healing..(3) in the early oftendon-bone healing compound of BMSCs with allogeneic bone and autologous tendon oftendon-bone healing is not significant difference with autologous bone and autologous tendonof tendon-bone healing in histology and biomechanical.(4) in the late of tendon-bone healingcomposite BMSCs allogeneic bone and autologous tendon of tendon-bone healing, notcomposite BMSCs allogeneic bone and autologous tendon of tendon-bone healing,autologous bone and autologous tendon of tendon-bone healing in biomechanical is botsignificant difference.(5) in the late of tendon-bone healing histology of observation favorablyto poor is followed by compound of BMSCs with allogeneic bone and autologous tendon oftendon-bone healing, autologous bone and autologous tendon of tendon-bone healing, notcompound BMSCs with allogeneic bone and autogenous tendon of tendon-bone healing. |
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