| Objective:Nm23gene (non-metsatatic23) is the metastasis suppressing gene of colorectal cancer isolated from the mouse melanoma K-1735cells by differential hybridization and other technologies. This paper is trying to explore the correlation between single nucleotide polymerphisms (SNP rs16949649T/C, rs2302254C/T) in nm23gene and CRC risk by case-control method.Method:182colorectal cancer cases were selected from The Fourth Ho-spital of Hebei Medical University as the objects during2008July to2009August, and200normal people were selected as health control.The experime-nt includes collecting5ml venous blood from the subjects and recording the information of their age, gender, smoking, alcohol consumption, exc. The method of protease K digestion and saturated sodium chloride salting-out was used to obtain the DNA in leukocytes of the objects. Polymerase Chain Reaction-Ligase Detection Reaction was used to determine the allele and the genotyping of the SNP point between rs16949649T/C and the rs2302254C/T of Nm23gene.Statistical analysis was finished by the SPSS13.0(SPSS Company, Chicago, Illionis, USA). t-test was used to analyze the age difference between the experimental group and control group;χ2test was used to deal with the gender differences, the allele frequency distribution and the genotype frequency distribution, they were also analyzed by Hardy-Weinberg equilibrium analysis, in control group. In this paper, the OR and its95%confidence interval was calculated by non-conditional logistic regression.Results:1. The rs16949649T/C and rs2302254C/T genotype frequency distribution obey the Hardy-Weinberg equilibrium in the control group (P>0.05). 2. The analysis of correlation risk between nm23gene rs16949649T/C polymorphism and CRC.The frequency of allele C and T in nm23gene rs16949649T/C site was34.6%and65.4%in experimental group, while it was44.5%and55.5%in control group,with a significant difference (P=0.005). The increasing frequen-cy of allele C in nm23gene rs16949649T/C polymorphisms (SNPs) would lower the risk of CRC (OR=0.660,95%CI=0.492-0.884);while C/C, C/T, T/T genotype frequency in case group were12.6%,44.0%and43.4%, compared to19.0%,51.0%and30.0%in the control group, with significant difference (P=0.017). CRC risk increased by carrying T/T genotype, while it decreased by carrying C/T, C/C genotype, compared with the T/T homozygote genotype carrier, the C/C, C/T genotype carrier had a lower risk of colorectal cancer (OR=0.590,95%CI=0.377-0.923; OR=0.459,95%CI=0.248-0.851). Influence of nm23gene rs16949649T/C polymorphism was not found on the crowd of smoking and drinking by stratified analysis.3. The analysis of correlation risk between nm23gene rs2302254C/T polymorphism and CRC.The frequency of allele C and T in nm23gene rs2302254C/T site was80.5%and19.5%in experimental group, while it was72.5%and27.5%in control group, with a significant difference (P=0.009). The increasing frequency of allele C in nm23gene rs2302254C/T polymorphisms (SNPs) would enhance the risk of CRC (OR=1.565,95%CI=1.114-2.198); while C/C, C/T, T/T genotype frequency in case group were65.9%,29.1%and4.9%, compared to53.0%,39.0%and8.0%in the control group, with significant difference(P=0.033). CRC risk increased by carrying C/C genotype, while it decreased by carrying C/T, T/T genotype, compared with the C/C homozygote genotype carrier, the T/T, C/T genotype carrier had a lower risk of colorectal cancer (OR=0.602,95%CI=0.389-0.931; OR=0.584,95%CI=0.386-0.885). Influence of nm23gene rs2302254C/T polymorphism was not found on the crowd of smoking and drinking by stratified analysis. Conclusion:1.The risk of colorectal cancer is related to the single nucleotide polym-orphisms of nm23gene rs16949649T/C, increasing risk related to carrying the T/T genotype, without effecting to the smoking and drinking crowd.2.The risk of colorectal cancer is related to the single nucleotide polym-orphisms of nm23gene rs2302254C/T, increasing risk related to carrying the C/C genotype, without effecting to the smoking and drinking crowd. |
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