Triamciolone Acetonide Effect On Fibroblast Of Human Benign Bile Duct Scar In Vitro

[Objective] To study the effcets of Triamcinolone Acetonide on the fibroblasts of human benign bile duct scar proliferation,apoptosis,expression of TGF-β1, alpha-SMA,Ⅰ and Ⅲ type collagen. To explore the mechanism and provide a theoretical basis of clinical application of triamcinolone acetonide treatment on human benign bile duct scar.[Methods] Recovery and cultivate the fibroblasts of human benign bile duct scar.Passages3-5cells were used for the experiments.They were divided into control group and triamciolone acetonide group. Detection the cells proliferation and the influence of triamciolone acetonide (Omg/ml,0.0625mg/ml,0.125mg/ml,0.25mg/ml,0.5mg/ml,1mg/mll,2mg/ml,4mg/ml,8mg/ml) on FB after24hours,48hours,72hours by MTT and apoptosis under half inhibition concentration (IC50) after72h by flow cytometric (Annexin V/PI double marking). Determination of expression of TGF β-1,alpha-SMA,Ⅰ and Ⅲ type collagen by cellular immune fluorescent chemical method and Western Blot method.[Results]①Human benign bile duct scar fibroblasts2days after resuscitation training covered bottom,was long spindle cells.②MTT assay of triamciolone acetonide in human benign bile duct scar flbroblasts affected were as follows:it can inhibit fibroblasts proliferation of human benign bile duct scar fibroblasts, and as the extension of time and the increase in drug concentration, the role of inhibition enhanced from0.0625mg/ml to8mg/ml of triamciolone acetonide. IC50of24h is6.71mg/ml,48h is4.33mg/ml and72h is2.72mg/ml,and take2.72mg/ml as expremental concentration.③Triamciolone acetonide can significantly promoted apoptosis of the fibroblasts of human benign bile duct scar. ④Triamciolone acetonide can significantly inhibited expression of TGF-β1and alpha-SMA,Ⅰ and Ⅲ type collagen.[Conclusions] Triamciolone acetonide can inhibit fibroblasts proliferation of human benign bile duct scar fibroblasts, and as the extension of time and the increase in drug concentration, the role of inhibition enhanced; and significantly promoted apoptosis of the fibroblasts of human benign bile duct scar; and significantly inhibited expression of TGF-β1,alpha-SMA,Ⅰ and Ⅲ type collagen for treatment of benign bile duct scar.