Discussing The Application Value Of Diagnoseing Enterovirus Central Nervous System Infection By The Genic Typing Method

Objective the aims of the report were to disscuse the clinic value of a new genic typing method in diagnoseing Enterovirus central nervous system infectionMethods we selecte 50 children who were diagnosed worrying viral encephalitis in our hospital from 2006.06-2009.08, drawing the CSF at the first day of hospitalization. Firstly,we selected the sample infected EV by a general RT-PCR amplification with a pair of primers designed by the 5'UTR of the EV genome. Secondly, we divide the selected samples into two groups. One group we amplify the VP1 region with a pair of specific primers to aim directly at this region.The other we amplify the central of the VP2 region with a pair of specific primers to aim at the region.Thirdly we sequence and subtype the amplified productions.The last we affirm the consequence of the subtype by using the monovalent antiserum corresponding to the type identified by sequencing essays.We also erect the positive control group and the negative control group in the essay.Results EV RNA were detected from 45 clinic samples and 3 positive control samples. Among the total, we successfully amplify the virus genome and gain a 357 bp GE strap by VP1 method from 38 clinic samples and a 584 bp GE strap by VP2 method from 34 clinic samples. By BLAST system contrasting, there is more concordance and complimentarity in the two methods.Conclusion①We can successfully amplify the most serotype of EV by the classic VP1 method in the clinic.②There is more sensibility and higher rate of typing by the VP1 and VP2 method than only by the classic VP1 method.It is worthly to generalize this method in the clinic.