Recombinant EGFP Lentiviral Vector Tracing Transfection Umbilical Cord Mesenchymal Stem Cells Combined With ¦Â-tricalcium Phosphate Transplantation Therapy Free Of Necrosis Of The Femoral Head Defects

Objectiveto observe the repair results of recombinant lentiviral vector tracing enhanced green fluorescent protein (EGFP) tracer-transfected mesenchymal stem cells in Umbilical cord mesenchymal stem cells,(UC-MSCs) combinedβ-tricalcium phosphate transplantation in rabbit of femoral head necrosis defect, find new ways a treatment for clinical treatment of femoral head necrosis.MethodUC-MSCs in vitro proliferation of bone morphogenetic protein -2 (BMP-2) gene recombinant plasmid carrying enhanced green fluorescent protein (EGFP) lentiviral vector, the combination of BMP-2 gene in recombinant plasmid carrying enhanced green fluorescent protein (EGFP) of the lentiviral vector and umbilical cord mesenchymal stem cells (UC-MSCs) were cultured; 24 rabbit models of femoral head defect were made and randomly divided into 2 groups,12 in each, A group:bone defect filled withβ-TCP, B group: bone defects filled with recombinant recombinant enhanced green fluorescent protein (EGFP) transfected with lentiviral vector tracing the umbilical cord mesenchymal stem cells (UC-MSCs)+β-TCP; New Zealand white rabbits were respectively killed 4afte implanted 4,6,8 weeks, MRI imaging of the femoral head and general observation, HE staining and newborn trabecular bone pathology Determination of area percentage.ResultsGeneral observation of femoral head, MRI and pathological examination HE showed group B after 4 weeks of bone defect phaseβ-tricalcium phosphate degradation, with a clear response and bone formation of new bone, cartilage does not fall off the femoral head, femoral bones do not collapse, at 8 weeks of basicβ-tricalcium phosphate completely degraded, the femoral head completely basic repair bone defects, a small amount of cartilage defects of femoral head cartilage defects of the femoral head does not collapse; A group of 4 weeks,β-femoral bone defect is gradual degradation of calcium phosphate filled and filled a small amount of fibrous tissue, the femoral head cartilage loss, bone defects surrounding bone sclerosis, bone mineral density increased.8 weeks, the femoral head defect basicβ-tricalcium phosphate degradation, sclerotic bone around the femoral head trabecular bone disorders, and deformation of femoral head collapse. Statistical analysis:using computer software to calculate bone defects 8 weeks after the bone trabecular area percentage value, the percentage of its average t-test area, over statistical analysis showed that, with homogeneity of variance, P<0.05. Overall there differences were statistically significant.ConclusionRecombinant recombinant enhanced green fluorescent protein (EGFP) transfected with lentiviral vector tracing the umbilical cord mesenchymal stem cells (UCB-MSCs) in the body have a stronger role in the induction of bone formation and bone defects can successfully repair the femoral head;β-phosphate Three calcium and mesenchymal stem cells grow well and have good tissue compatibility and biodegradability, can be used as a good bone scaffold.