Effect Of Fasudil On The Inflammatory Cytokine Expression In Rats After Acut Myocardial Infarction

Objective:To explore the effect of fasudil on the inflammatory cytokine expression in rats heart following acut myocardial infarction(AMI)and its relationship with the Rho kinase.Methods:The model of AMI rats were divided into two groups, treated group(F group) and controled group(C group),besides another group of sham -operation(S group). Fasudil 5mg/kg were given into the treated group twice a day. The controled group and sham -operation group were given equivalent normal saline. Nikon 4 multi-channel physiological recorder records hemodynamics parameters.Radioimmunoassay detected Serum cytokines. EvensBlue and NBT staining identify ischemia and infarction area. The Rho kinase mRNA expression was measured by RT-PCR.Results:Compared with the sham -operation group, controled group of TNF-α,IL-1βand left ventricular end diastolic pressure (LVEDP) expression are significantly higher,the left ventricular systolic pressure (LVSP) and left ventricular pressure rise/fall maximum rate (±dP/dtmax) are significantly lower (P<0.05). With the controled group, treated group significantly reduced infarct size, TNF-αand IL-1βand Rho-kinase mRNA expression levels. Treated group is more significant reduction in LVEDP, increased LVSP and±dp/dtmax (P<0.01), left ventricular function improved significantly..Conclusion:Fasudil reduces myocardial infarction myocardial Rho kinase and inflammatory cytokine expression, improve heart function. Fasudil reduced the expression of inflammatory factors and improve cardiac function may be related to inhibition of Rho kinase activation..