Effect Of Psychological Stress On Infiltration, Metastasis In Pancreatic Cancer And Detection Of β2-adrenergic Receptor

Objective To investigate the relationship with stress and infiltration,metastasis of pancreatic cancer by detecting the expression of ofβ2-adrenergic receptor (β2-AR) in pancreatic cancer. Methods①Establish restraint stress model of human pancreatic neoplasm in nude mice, the 20 mice were randomly divided into the following two groups (n=10/group) respectively:the restraint stress model group and the control group. PANC-1 pancreatic cells in the logarithmic phase were injected subcutaneously into the right shoulder of the mice.The control group was provided with free access to food and water. The restraint stress model was established by putting each nude mouse respectively into a self-made tube with a 5mm hole for excellent ventilation. The mice were not squeezed their tails and could move in the tubes. Eight hours later, the animals were released from the tubes and then supplied with food and water. During the study the mice of restraint stress model were restrained 8h/day for two weeks. The serum epinephrine level were detected with enzyme-linked immunosorbent assay(ELISA). The expression ofβ2-AR was detected by immunohistochemical method and RT-PCR.②The expression ofβ2-AR was detected by immunohistochemical method in 40 clinical cases of pancreatic cancer tissues and 10 cases of normal tissues, The differences were statistically evaluated betweenβ2-AR expression and various clinicopathological factors in pancreatic cancer tissues. Results①The serum epinephrine level in transplanted tumors of the model group was higher than the control group, and the difference was statistically significant (t=-2.812, P<0.05).②As to the expression ofβ2-AR in transplanted tumors, the model group had a higher expression level compared with the control group in both level of protein and mRNA, and the difference was statistically significant (P<0.05).③The positive expression rate ofβ2-AR in pancreatic cancer tissues and normal tissues was 77.5%(31/40) and 40%(4/10) respectively, The difference between them being statistically significant (Z=-2.475, p<0.05). The positive expression ofβ2-AR in pancreatic cancer tissues was correlated with TNM staging and metastasis of lymph node (Z=-3.372～-3.073, p<0.05), but not with gender, age of patients, tumor size and tumor differentiation (Z=-1.395～-0.060, P>0.05). Conclusion Stress plays an important role in infiltration and metastasis of pancreatic cancer through influence the expression ofβ2-AR, Drugs to blockβ2-AR might be used for the prevention and treatment of pancreatic cancer.