Pulmonary Alveolus Syringe Fluid CD4+/CD8+ To Lung Sarcoidosis's Diagnosis Value

Purpose:The establishment (FCM) examines the pulmonary alveolus syringe fluid by the class type cell meter (BALF) lymphocyte CD4+/CD8+to the lung sarcoidosis's diagnosis value.Method:Use of FITC-CD45/PE-CD14 equipment and unify the PI dyeing to remove the non-lymphocyte, the cell fragment and so on to the examination disturbance, analyzes 30 example lungs illness sickness patient BALF by FCM, choice case double lung CT has the lung hilus, the mediastinum lymph node tumescent merge or non-lung internal illness changes the line of TBNA biopsy, the cellular pathology is clear about the diagnosis. With the fashionable pulmonary alveolus syringe fluid CD4+/CD8+inspection, observes pulmonary alveolus syringe fluid CD4+/CD8+at the lung sarcoidosis and between the lymph node tuberculosis difference.Result:14 examples after TBNA biopsy diagnosis sarcoidosis:The cell surface differentiation antigen (CD3) and the CD4 cell increases obviously, the CD8 cell number obvious drop, the CD4+/CD8+ratio elevates, CD4+/CD8+is bigger than 3.512 examples, other 2 examples respectively are 3.0,2.8. The TBNA diagnosis for the lymph node tuberculosis patient 16 example T cell subgrouConclusion:Pulmonary alveolus syringe fluid CD4+/CD8+has the important reference value to the lung sarcoidosis and the lymph node tuberculosis distinction. sarcoidosis patient's BAL demonstration lymph cellularity pulmonary alveolitis. Usually the sarcoidosis patient's lymphocyte CD4+/CD8+ratio elevates and the lymph node tuberculosis patient, obviously difference. has the important reference valueaccounts for 90%, the sarcoidosis patient's lymphocyte accounts for 30%. Outside some chests sarcoidosis like iris eyelash shape body inflammation, although the chest phantom study is normal, but the BAL prompt lungs implicate.