The Detection And Research Of Serum P53 Antibodies In Cancer Patients And Check-up Population With ELISA Method

Cancer has been the most dangerous threat to human health and the top cause of death in city and countryside of China. In China, approximately 2.6 million people were diagnosed with cancer every year, and about 1.8 million persons died. The reasons for this phenomenon varied. For example, tumor formation is complex process. However one of the most important reasons is that there is still no reliable way to detect and prevent cancer before it has begun its insidious insurrection, especially early dectection. Early diagnosis appears an especially important strategy as there is no effective method to overcome this disease at present. The study found that p53 antibodies found in various types of cancer,has the character of the specificity and can be used as an important specific tumor maker for the early detection.In this paper, we prepared recombinant full-length human p53 protein and hybrid phage displaying DO7 epitope of p53 protein. Using fusion p53 protein, hybrid phage displaying DO7 epitope of p53 protein and the mixture of p53 protein and hybrid phage as coating antigen respectively, we detect 316 cancer patients and 874 teachers and staff members by ELISA. The results are as follows: 1) Using the full-length p53 protein as coating antigen, the positive rate of serum p53-Abs in the cancer patients was 20.3%. 2) Using the hybrid phage protein as coating antigen, the positive rate of serum p53-Abs in cancer patients was 18%. 3) Using the mixture of p53 protein and hybrid phage as coating antigen, the positive rate of p53 Abs in cancer patients was 25.9%. 4) Using three sets of ELISA procedure to detect serum p53-Abs of 874 persons, 37 persons were found serum p53-Abs positive and monitored and observed furthermore.