Effection Of RhG-CSF On Proliferation And Apoptosis Of MCF-7 Human Breast Cancer Cells

Objective:Human recombinant colony-stimulating factors may be used to treat or prevent neutropenia caused by marrow toxic chemotherapeutic agents administered to patients with breast cancer. To evaluate the effection of RhG-CSF on breast cancer, we cultures the cells one of The highest incidence breast cancer cell lines(the MCF-7) with RhG-CSF over a wide range of pharmacologic doses.Methods: We cultures the cells by contains RhG-CSF cell cultures.And the concentration of RhG-CSF was 0.1ug/l,1.0ug/l,10ug/l,100ug/l and l000ug/l. After 12h,24h,48h,72h,96h,and 120h,we test the cells. The MCF-7 cell growth curve was drawn by (MTT) assay.Flow cytometry (FCM) was adopted to analyze quantitatively the cell cycle and the cell apoptosis.Results: As compared with MCF-7 cells cultured with DMEM,the cells cultured with RhG-CSF was markedly eugonic (P<0.01),and the largest eugonic effect was seen at 10ug/l.Cell cycle study found that cell cycle at S phase after 24h exposure to RhG-CSF compared with the negative control group(P<0.01).Moreover,apoptotic cells which cultured with RhG-CSF was also decreased(P<0.01) ; Conclusions:RhG-CSF can significantly promote MCF - 7 cell proliferation. These changes was demonstrate statistical significance at different doses. Along with the extension of cultured time, the proliferation was stronger.And the growth in a dose-dependent fashion when the doses less than 10ug/l, more than 10ug/l, it was contrary .The proliferation is consider that the RhG-CSF increased cells at S phase .What about Apoptosis rate was decreased compared with control group after 24h.