The Research Of Exogenous Creatine Phosphate To Relieve Acute Exercise Fatigue And The Protection To Nerve Cell In Hypoxia

Objective :With the development of professional sports and the increase of civil sports, a lot of attention has been paid to the sports fatigue by international experts. However, the systematic study of sports fatigue mainly started since 1970s. By now creatine, as the energy supplement and storage in competitions, mainly promotes the compound of phosphate and muscle, which can prevent and eliminate sports fatigue and keep athletes in good condition during training and competition. But it can bring some side-effects after a long time use such as muscle stiffness, soreness and weight growth. CP applies to the re-compound of ATP directly which can offer energy for our body. Clinically, CP has many applications such as Myocardial infarction, Heart failure, any cardiac surgery, which can protect Myocardial ischemia and reduce the chances for Ventricular arrhythmia occurrence. But it is hardly seen in reports that exogenous CP has been used as energy supplement in competitions. In this research we use Kun Ming mouse and its Neuro2A cells as research objects ,in the overall level of animal and cell level to find out anti-fatigue effects by the exogenous CP to relieve acute exercise fatigue and the protection to Nerve cell in hypoxia.Methods:(1)36 Male Kun Min mice, 2-month-age, weight 22±3g, temperature 25±2°C, relative humidity 50±5%. The mice were fed separately for 9 mice in one cage supplied by natural sunshine, free food and water. 3 days later divided them into 3 groups: A--control group, 12 mice; B—swimming group, 12 mice; C—dosing swimming group, 12 mice. For A group, no sports, free food and water. let B group and C group take part in 3-day adaptive swimming training with water temperature 28±1°C and dried up the mouse's hair after training. On the 7th day, injected B group with Intraperitoneal of saline, injected C group with CP. After the injection let them swim until being exhaustive, then killed them, got the heart blood and make them centrifugal rapidly. Then got their hearts and brachial quadriceps into the fridge stored under -80°C. Methods of CK Kit, No Kit and NOS Kit were used in Biochemical tests. All data were carried out by SPSS 11.5 statistical software.(2)Using the Trypsin, we digested the logarithmic phase Neuro2A cells. Then tiled them on the 96 orifice, poured into different concentrations of CP, protecting well under the hypoxia 20mins, testing the survival rates of every role with MTT method.Result :(1)The exercise time for dosing (CP) group C is apparently longer than group B. The index figure of NO, NOS, CK of group B is apparently higher than group A(P<0.01). The index figure of NO, NOS, CK of group C is apparently lower than group B(P<0.01)but still higher than group A(.2)The survival rates of Neuro2A cells for 10μmol/ml group is obviously higher than other groups.Conclusion :CP can enhance the exercise capacity of mouse noticeably, as well as lower the oxidative stress effect to the body from free radical and the protection to Nerve cell in hypoxia.