| Objective: Neovascular glaucoma (NVG),one of the important reasons of blindness, is a complex etiology, clinical presentation of refractory severe intractable glaucoma. Posterior segment is usually secondary to a wide range of ischemic diseases such as diabetic retinopathy (DR), retinal vein occlusion (RVO) and so on. The main clinical features are high intraocular pressure and severe eye pain which are difficult to control , while conventional glaucoma treatment be hard to work, ultimately often leads to severe loss of visual function and other serious consequences even enucleation. There are no effective clinical treatments to NVG, so early diagnosis, prevention and treatment to take positive and effective measures are the key to control intraocular pressure and keep a better vision. At present, the specific pathogenesis for the NVG is not clear, but studies at home and abroad has never stopped . In recent years, with the development of molecular biology and immunology, a number of angiogenesis-related factors and their receptors on cells have absorbed more and more scholars. Vascular endothelial growth factor (VEGF) is a kind of specifically stimulate endothelial cell proliferation and angiogenesis in platelet-derived protein. Interleukin -6 (IL-6),a multi-functional regulation of inflammatory cytokine response, is the the main inducing factor of acute stage of infection or trauma stress. This study was designed to further clarify the vascular growth factor on the incidence of NVG by detecting the NVG serum and aqueous humor VEGF and IL-6 levels observed between them and their relationship with the NVG, to reveal the possible pathogenesis of NVG ; and try to find a response in the serum indicators of intraocular fluid situation the serum and aqueous humor by comparing the correlation between vascular factors, to provide a new theoretical basis of clinical evaluation and prognosis of the disease . Methods: 20 patients with NVG were selected as experimental group (group A), 20 patients with primary chronic angle closure glaucoma patients (group B), 20 cases of senile cataract patients (group C) as a control group. Collected three specimens of serum and aqueous humor, through the double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay, ELISA method) were measured in serum and aqueous humor VEGF, IL-6 concentrations. Comparison of VEGF, IL-6 levels in each group changes; specimens of each group was analyzed VEGF and IL-6 in the correlation; analysis in the serum VEGF, IL-6 levels and differences in aqueous concentrations and their relationship. Application SPSS17.0 statistical software to process data, the same group the same factor in comparison of data between different samples with independent samples t test. All data have homogeneity of variance, each factor between the different groups compared by analysis of variance (ANOVA). Correlation analysis using Pearson correlation coefficient test. P <0.05 was considered statistically significant.Results: 1. Each group VEGF, IL-6 concentration levels:①the concentration of VEGF in aqueous humor: group A (1336.80±70.15) pg / ml was significantly higher than that of group B (311.60±31.06) pg / ml and the group C (165.75±13.95) pg / ml, among the three groups, both the differences of pairwise comparison were significant (F = 4019.334, P <0.05).②IL-6 levels in aqueous humor compared: group A (691.15±50.09) pg / ml was significantly higher than that of group B (168.25±11.95) pg / ml and the group C (92.10±9.59) pg / ml, pairwise comparisons among the three groups , the differences were statistically significant (F = 2275.019, P <0.05).③the concentration of serum VEGF: group A (545.40±155.49) pg / ml higher than the group B (321.15±52.57) pg / ml and the group C (176.30±20.38) pg / ml, pairwise comparison among the three groups, the difference was statistically significant (F = 75.940, P <0.05).④serum concentrations of IL-6: group A (291.35±22.66) pg / ml higher than the group B (104.35±13.21) pg / ml and the group C (87.00±12.70) pg / ml, pairwise comparison among the three groups, differences were statistically significant (F = 906.947, P <0.05).2. NVG group of aqueous VEGF and IL-6 concentration showed a significant positive correlation, statistically significant difference (r = 0.857, P <0.05). The remaining specimens in each group no significant correlation (P> 0.05).3. Each group of serum VEGF, IL-6 concentration and the corresponding aqueous humor was no significant correlation (P> 0.05).4. NVG patients aqueous humor VEGF, IL-6 were significantly higher than that of serum, the difference was statistically significant (t =- 20.748, P <0.05; t = -32.199, P <0.05).Conclusion: 1. VEGF, IL-6 may promote the development of NVG.2.VEGF, IL-6 play the role of coordination with each other during the formation of the NVG.3. There may be VEGF, IL-6 production and independent mechanisms in the ocular of NVG. |
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