| Background:Atherosclerosis (AS) is a severe disease that has great affects on the health of human being. The research on its mechanism has been going on for several decades. It is confirmed that the inflammation and endothelial dysfunction play an important role in the occurrence and development of AS. Cellular signal pathways can be stimulated by a lot of risk factors, which promote the development of AS. Renin-angiotensin system (RAS) is one of the main regulators of cardiovascular diseases. Over activity of RAS can accelerate the formation of AS by increasing blood pressure, oxidative stress, inflammatory response and endothelial dysfunction and so on. In the classical RAS, renin has been considered to convert the angiotensinogen to angiotensinⅠand angiotensinⅠcan convert to angiotensinⅡwhich plays an important part in several diseases. Other than that, renin has no other biological action. However, as a new molecular of RAS, prorenin receptor (PRR) has provided us the better and further understanding of prorenin's function. Several complex biological responses such as renal mesangial cell proliferation, mesangial matrix accumulation and vascular smooth cell migration result from the combination between receptor and ligand. In animal models , these reactions lead to elevated blood pressure, proteinuria and glomerulosclerosis. Recently, our research group has found that the prorenin receptor can be expressed in human umbilical vein cell and proved its signal pathways and the relationship between endothelial dysfunction and AS is further confirmed.High glucose is considered as one of the most important risk factors of AS. The expression of glucose transporters on the surface of cell can be up-regulated under high glucose environment. After that, some signal pathway is activated and inflam-matory factors is over-expressed. Some groups have reported that high glucose mediate renin, prorenin, angiotensinogen, angiotensin-converting enzyme increase in the human mesangial cell, but the effect in the human umbilical vein cell(HUVECs) is unknown. The purpose of this study is to observe the change of prorenin expression in HUVECs under hypothesized high blood sugar environment.Methods:1. HUVECs were cultured in vitro.2. Choose HUVECs in good condition, divided them into 4 groups: control, normal glucose group, high glucose group1 and high glucose group2. The concentration of glucose is 0mM, 5mM, 25mM, 40mM respectively to observe the effect of glucose on the expression of prorenin mRNA in HUVECs.3. In order to observe the effect of osmotic pressure, HUVECs were treated by mannitol 0mM, 5mM, 25mM and 40mM respectively to observe the prorenin expression.4. HUVECs were co-cultured with high glucose(40mM) in 0day, 1day, 2days, 3days, 4days, 5days, 6days respectively, to observe the prorenin expression after different times.5. Prorenin mRNA was evaluated by RT-PCR.6. The concentration of prorenin in cultural medium was evaluated by ELISA kit.Results:1. Comparing to the control group, normal dose of glucose can't upregulate the prorenin mRNA expression; high glucose induce the prorenin mRNA over-expression, especially in the 40mM.2. There were no difference of prorenin mRNA expression in the different concentration of mannitol groups.3. The expression of prorenin mRNA increased time-dependent obviously under 40mM glucose.4. In cultural medium, prorenin expression were increased and showed time-dependently.Conclusions:1. High glucose can increase the expression of prorenin mRNA increase express in HUVECs concentration-dependently.2. The effect of osmotic press can be ignored.3. High glucose can stimulate the prorenin mRNA express time-dependently in HUVECs. 4. High glucose can make the expression of prorenin time-dependently in HUVECs. |
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