Analysis On Effects Of Expression Of MMPs And ECM By Hormonal And Correlation Of β1,4-GalTⅠ

Objective: During implantation that includes cell recognition and cell adhesion,there are many correlation factors regulating implantation , such as hormone,cytokine,growth factor and receptor,proteinase,glycans and so on. Estrogens can make endometrial development,hyperplasy and follicle maturation. Progestogens can make endometrial transform from estrin phase to secretory phase and adoption phase.In the article,we shall study the associativity between correlation factors of implantation especially MMPs and ECM andβ1,4-GalTⅠwhen adoption phase of endometrial raises.Methods: We assemble clinical endometrial specimen of estrin phase and secretory phase to analyze allocation and expression associativity among MMPs,TIMPs,ECM andβ1,4-GalTⅠ.We simulate physiological process of estrogens and progestogens in vivo and prepare mouse model in order to view expression changes of MMPs,ECM and TIMPs in endometrial after the level of hormones is changed. Regulatory role of the hormone and its associativity to the expression ofβ1,4-GalTⅠcan be further clarified .Results: Immunohistochemistry shows that MMPs(MMP-2,MMP-9)express in endometrial epithelial cells and interstitial cells, and express higher in secretory phase than follicular phase.We change the level of hormone and MMPs express as follow: the expression and activity of MMP-9 in hormone group is lower than normal group and control group.However,if we just think over the roles of hormone, progestogens make MMP-9 up-regulation and estrogens make MMP-9 down-regulation,while progestogens make MMP-2 up-regulation obviously. Progestogens and estrogens both make MMP-9 up-regulation. Active gelatinase-B(MMP-9,84KDa)and gelatinase-A can be made an fiercer influence by progestogens than estrogens. The results show progestogens mainly regulate the expression and secretion. FN and LN express in endometrial interstitial cells higher in follicular phase than secretory phase.TIMP-1 express notabley in endometrial epithelial cells of follicular phase and secretory phase. Progestogens restrain TIMP-1 and FN gene expression and secretion of proteins of FN and LN,and estrogens also make down-regulation. Progestogen regulate MMPs in implantation period up-regulation ,which degradate basilar membrane and matrix and promote embryo implantation and the formation of neovessels.β1,4-GalTⅠmainly expresses in local interstitial cells of endometrial secretory phase. Estrogens promoteβ1,4-GalTⅠexpression and protein excretion. Progestogens makeβ1,4-GalTⅠdown-regulation, while estrogen group and associated group makeβ1,4-GalTⅠup-regulation in protein excretion .Over-expression ofβ1,4-GalTⅠcan promote MMPs expression and secretion,and make an effect on adhesion rate between JAR cells and RL95-2 cells. Expression of MMPs in implantation period increases,while that of ECM decreases. The level of MMP-TIMP makes dynamic balance in some extent , which is easy to selectively hydrolyse endometrial stroma and which regulates trophoblastic cells invasion.β1,4-GalTⅠregulates recognition and adhesion between matrix and embryo ,which is correlated with synthesis of MMP-9,and which mediates embryo nidation together by hormonal.Conclusion: The expression of MMPs is regulated by hormonal,and progestogens can make expression of MMP-2 and MMP-9 increase and that of FN and LN decrease in implantation period (secretory phase ).Both progestogens and estrogens regulate expression of MMPs,ECM and TIMPs together in order to maintain dynamic balance.β1,4-GalTⅠregulates recognition and adhesion between matrix and embryo ,which is correlated with synthesis of MMP-9,and which mediates embryo nidation together by hormonal.