Research On The Preparation And Property Of The Pine Needle

The task's material is from pine needle of cedar,making a systematic study and analysis about extraction,hydrolysis,purification,stability and so on of pine needle polyprenols and acetic ester,providing theoretical reference and scientific basis.The main conclusions are as follows:The content of pine needle polyprenols and acetic ester is different on different months,the content from September to November is higher.The beauty extracting technology by single factor and response surface method is:petroleum is solvent,ultrasonic as extracting method,ratio of solid to liquid is 8.5g/mL,ultrasonic power is 90W,ultrasonic time is 62min,ultrasonic temperature is 42℃.The hydrolysis condition of pine needle polyprenol acetic ester is:5%NaOH-CH₃OH solution,hot reflux magnetic force stirring 2.5h on 30℃. The optimization condition of dewaxing on the extractum of pine needle polyprenols is:adopting acetone 1:10g/mL:acetic ether(9:1)on 0℃.The purification of pine needle polyprenols is by the method of adsorption chromatograph,the technological parameter is:100-200 silica gel,firstly eluting by petroleum ether,secondly eluting by petroleum ether:acetic ether(19:1),the flow velocity is under 1 mL/min.Thin layer chromatography detection,colleting polyprenols,reduced pressure distillation,vacuum and freeze drying for the sample of polyprenols.The pine needle polyprenols have five monocases,whose M of isopentene group is twenty three,twenty one ,twenty,eighteen,fifteen.The effect of temperature on pine needle polyprenols is notability:when temperature is under 30℃,the change of pine needle polyprenols'absorbance,the stability is better,therefore,the conservation of pine needle polyprenols should be under 30℃;the illumination make an effect on pine needle polyprenols stability,which should be conserved on darkness;the pine needle polyprenols is sensitivity on different pH : strong acid and strong alkali is notability to pine needle polyrenols,pine needle polyprenols is stability on neutral or weak acid and alkali.The effect of metal ions on pine needle polyprenols:the effect of pine needle polyprenols stability is little by Ca²⁺,Cu²⁺,Al³⁺,Zn²⁺,K⁺,Mg²⁺;the effect of pine needle polyprenols stability is much notability by Fe³⁺,Fe²⁺,Ag⁺.The polyprenols is belong to lipoids,whose acid value,iodine value and saponification value of pine needle polyprenols is 3.89 mg KOH/g,2.65 mg KOH/g,2.06 mg KOH/g;The clearance of pine needle polyprenols is up to 23.1% by the method of pyrogallol auto-oxidation.