Effect Of VEGF Gene Blocked By RNAi On B Cell Malignant Lymphoma

Objective: To explore the effect of RNA interferencemediated vascular endothelial growth factor (VEGF) gene silencing on biological behavior of Namalwa cells. Methods : Three specific siRNAs targeting to VEGF were synthesized and transfected into B cell malignant lymphoma Namalwa cells by lipofectamine2000,according to the principle of target sequence of siRNA.After dssiRNA was transfected into Namalwa cells with Lipofectamine, the proliferative and invasive ability of Namalwa cells was evaluated by transwell migration assay and MTT. The expression of VEGF on mRNA and protein level was analyzed by RT-PCR and Western Blot technology ,respectively. Results: In the three siRNA targeting human VEGF gene,only si-2group have statistical significance (p<0.01 ) compared with negative control siRNA (siRNA-NC) group and normal group ,it effectively inhibited the proliferation of Namalwa cells, and decreased the expression of VEGF on both mRNA and protein level. But these effects did not appear in negative control siRNA (siRNA-NC) group. Conclusions: The siRNA targeting human VEGF gene could reduce VEGF expression, inhibit cellular proliferation and induce apoptosis in Namalwa cells in vitro, suggesting that siRNA-based strategy targeting VEGF may lay a foundation for the clinical management of B cell malignant lymphoma.