The Protective Effect Of Hydrogen-Rich Saline On Isoproterenol-Induced Acute Myocardial Infarction In Rat

Background and ObjectiveThe recent studies have confirmed that free radical damage plays a very important role in the pathogenesis of acute myocardial infarction. Hydrogen has been reported to act as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. This research was designed to investigate the protective effect of hydrogen-rich saline (HRS) on acute myocardial infarction (AMI).MethodsModel of AMI was induced by subcutaneous injection of ISO (200 mg·kg^-1body weight in 1 ml saline) for 2 consecutive days in male Wistar rats (n=60, 180-220g) which were randomly divided into six groups: Control, Model, HRS (ip.5 ml·kg^-1), HRS (ip.7.5 ml·kg^-1), HRS (ip.10 ml·kg^-1) and Vitamin C (ig. 250mg?kg-1). Serum CK-MB,AST, SOD, MDA, 8-OH-dG, IL-6, TNF-α, and heart tissues SOD, MDA, 8-OH-dG, IL-6, TNF-α, Ca²⁺-ATPase and Na⁺-K⁺-ATPase. TTC test was used to analyze macroscopic changes of infarct size of heart tissues and HE staining to observe the pathological changes.ResultsCompared to normal controls, serum CK-MB activity,AST content and infarct size rose significantly in model group. Compared to model group, CK-MB activity,AST content, the infarct size, serum and tissue MDA,8-OH-dG,IL-6,TNF-αand Ca²⁺-ATPase levels all lowered significantly, and serum and tissue SOD and Na⁺-K⁺-ATPase activity were increased significantly upon pretreatment with HRS and Vitamin C. ConclusionsIt is concluded that hydrogen-rich saline exerts a protective effect against ISO-induced AMI possibly by antioxidative stress and antiinflammation.