The Impact Of Different Culture Mediums System On Mouse Oocytes Parthenogenetic Activation

Objective This study takes parthenogenetic embryo and normal fertilization embryo of Caesarean Derived-1mouse as test materials to explore the influence that different culture mediums and vitro cultivating environment have made to mouse parthenogenetic embryo and normal fertilization embryo.The purpose is to find a proper kind of cultivation liquid and environment.To study the impact of the same cultivation liquid on the development of both parthenogenetic embryo and normal fertilization embryo.To discuss the nutrient content of different development stages of fertilization ,merogensis,blastula of parthenogenetic embryo.This will not only help improving the methodology of parthenogenetic embryo experiment approach,but also improve human cultural method.At the same time ,it could be taken as a reference for the in vitro fertilization-embryo transfer.Methods Three different types of culture mediums(1604,Irvine,Vitrolife )are selected artificially to cultivate parthenogenetic embryo and normal fertilization embryo respectively. The analysis of blastula formation rate,cleavage rate and blastula hatching rate are used to compare the developmental rate of parthenogenetic embryo and normal fertilization embryo. Through the observation of the blastula formation rate,cleavage rate and blastula hatching rate, the impact should be figured down on different developmental periods of different parthenogenetic embryo and normal fertilization embryo,by the three differents types of culture mediums.Results There are significant different(P<0.05)between cleavage rate (58.1%,78.5%,81.7%),blastula formation rate(9.0%,36.5%,39.3%) and blastula hatching rate(0.6%,4.3%,7.3%) within 1604,Irvine and Vitrolife. Activation ovum rate (96.1%,94.2%,95.3%)of three different types of culture mediums are not remarkable different (P>0.05).There are no significant difference on blastula formation rate,cleavage ra te and blastula hatching rate between Irvine and Vitrolife (P>0.05).However,th embryo development of parthenogenetic embryo Vitrolife is better compared to Irvine.The cleavage rate, blastula formation rate and blastula hatching rate of 1604 are all lower than Irvine and Vitrolife,which is meaningful in statistics.Conclusion In the above three culture mediums discussed above:(1) Vitrolife culture mediums and Irvine culture mediums are better than 1640 culture mediums in terms of blastula formation rate,cleavage rate and blastula hatching rate of parthenogenetic embryo and normal fertilization embryo which means it is more suitable in parthenogenetic embryoexperments. (2)Same culture mediums have no significant difference on parthenogenetic embryo and normal fertilization,which means parthenogenetic embryo and normal fertilization have similar development potential.(3)The experimental results of the three different types of culture mediums in normal fertilization embryo and parthenogenetic embryo are note significant.