The Radiosensitization Of Aidi Injection To The Different Human Malignant Cell Lines And Corresponding Mechanisms

Objective: To evaluate the effect of Aidi injection in enhancing the radiosensitivity of human cancer cells and elucidate the underlying mechanisms in SHG44 cell lines from glioma.Methods: The proliferation of the H460 cell lines,the SHG44 cell lines and the HepG2 cell lines were measured by MTT assay; Cell survival was determined by cloning assay; The SHG44 Cell cycle distribution and apoptosis were analyzed by flow cytometry; Western blotting was used to determine the expression of CyclinB1 and Wee1 protein. Western blot assays were analyzed by densitometry software (Quantity One).Results:1. The inhibition rate in SHG44 cell line which was treated with 3mg/ml to 192mg/ml Aidi injection for 48h was different, and the same results in H460 and HepG2 cell lines.2. Cell survival experiment showed r adiosensitization of Aidi injection to the three kinds of human malignant cell lines. In SHG44 cells, The mean lethal(D₀) and quasithreshould dose(D_q) were 2.14 and 1.58 in R group, but 1.50 and 1.09 in 48h D15mg/ml+R group, sensitivity enhancement ratio(SER_D0) and SER_Dq were 1.43 and 1.45; In H460 cells, D₀ and D_q were 2.02 and 1.72 in R group, but 1.72 and 1.26 in 48h D12_mg/ml+R group, SER_D0 and SER_Dq were 1.17 and 1.37; In HepG2 cells, D₀ and D_q were 2.02 and 0.74 in R group, but 1.75 and 0.62 in 48h D12_mg/ml+R group, SER_D0 and SER_Dq were 1.15 and 1.20.3. FCM analysis showed that 15mg/ml Aidi injection enhanced prolongation of G2/M arrest which induced by irradiation 6Gy (R and D+R:G2/M: 16.77%±1.10 and 26.86%±1.37,P<0.01), and apoptotic cell death was induced also (R and D+R:11.5%±0.53 and 32%±2.2,P<0.01).4. The expression of CyclinB1 protein decreased in the group treated with irradiation and Aidi injection compared with irradiation or Aidi injection, while Wee1 protein increased; G2/M proportion had negative correlation with the CyclinB1 protein expression(r=-0.945,P<0.05), but had positive correlation with Weel protein expression(r=0.941,P<0.05).Conclusions:1. The radiosensitivition of Aidi injection was universal in SHG44 cells, H460 cells and HepG2 cells.2. Aidi injection had radiosensitization and its mechanism might be related to increase G2/M phase arrest and apoptotic cell death, down-regulate cyclinB1 protein and up-regulate Wee1 protein expression.3. The G2/M phase was prolonged by aidi injection in SHG44 cell which was related to down-regulate cyclinB1 protein expression and up-regulate Wee1 protein expression.