| Objective:To explore the method of quickly distinguishing between different states of the intestinal flora, use flight mass spectrometry to analyse intestinal flora protein of antibiotics interfered mice comparative with the normal mice. And to analyse intestinal flora protein of persons with bacteria-related diseases, such as ulcerative colitis, 2 diabetes and obesity, comparative with healthy ones.Methods:1.Experimental group and cases selection⑴Babl / c mice were randomly divided into two groups, the antibiotic interfered group and the control group, 5 each group. The former ones were fed with 400 mg/ml ceftriaxone sodium with 0.2 ml per time, 2 times per day, 6 h interval, and for 8 days. The later ones were fed with sterile water.⑵Clinical experiments were divided into four groups, the healthy group, UC group, type 2 diabetes and obese group.Health group 13 cases, aged from 25 to 61 years old, confirmed the healthy by clinical examination, with no bowel disease, and not taking any antibiotics within 3 months.Ulcerative colitis (UC) group 13 cases, meeingt the diagnostic criteria for ulcerative colitis, aged from 22 to 71 years old.Diagnostic criteria for ulcerative colitis: Clinical manifestations were persistent or recurrent episodes of diarrhea, mucus, pus and blood stool with abdominal pain, tenesmus, and varying degrees of systemic symptoms. The course of disease is more than 4-6 weeks. The auxiliary examination mainly is colonoscopy examination. 2 diabetic group 12 cases, meeting the diagnostic criteria for type 2 diabetes, aged from 34 to 80 years old.2 diabetes diagnostic criteria: Symptoms of diabetes is with plasma glucose≥11.1mmol/L at any time or FPG≥7.0mmol/L or OGTT 2h PG≥11.1mmol/L. Obese group 14 cases, according to body mass index (BMI) and physical examination for diagnosis, the BMI> 26 as the main indicator to judge obese, aged from 27 to 70 years old.2.Flight mass spectrometryUse the flight mass spectrometry to distinguish the intestinal flora protein profiles between the antibiotics interfered group and the control mice group. And the distinction the intestinal flora protein profiles among UC, type 2 diabetes, obese patients and the healthy.Results:1.Compared with the control group, the number of effective protein peak(m / z> 4500) of intestinal flora bacteria in the antibiotics interfered group significantly decreased(p<0.05). And the total number of valid peaks in two groups did not significantly change2.Compared with the control group, the number of effective protein peak of intestinal flora bacteria in the UC group significantly decreased(p<0.05). There were three sustained expression specific protein peak in the effective protein peaks of UC group, such as 3371.98, 3443.08, 3469.49 m/z. And their expression were significantly different from the normal control group(P<0.05). The probability of the three protein-specific peaks in the UC group is 100%.3.Compared with the control group, the number of the effective protein peaks of intestinal flora bacteria in the Obesity group was no significantly different.4.Compared with the control group, the number of the effective protein peaks of intestinal flora bacteria in the 2 diabetic group was no significantly different.Conclusion:1.There were significant difference of the intestinal microflora protein components between the antibiotics interfered and the normal mouse.The flight mass spectrometry can be quickly distinguished.2. There were significant difference of the intestinal microflora protein components between the UC and the healthy group. The flight mass spectrometry can be quickly distinguished.3.There were no significantly different of intestinal microflora protein components among type 2 diabetes, obesity protein and the healthy group. The flight mass spectrometry can not distinguish. |
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