Pharmacological Study Of Sodium Fulvate

Humic acid (HA) is produced by biodegradation of plant residues in nature, it is a complex polymer containing carboxyl, carbonyl, quinonyl, phenolic hydroxyl and other active functional groups. It can be divided into three main fractions by the molecular weight and solubility in different solvent:Black humic acid, Hymatomelanic acid, fulvic acid. Thousands of years ago, HA had been recorded in "Compendium of Materia Medica" compiled by Li Shizhen, which was mainly used for the treatment of nose bleeding, women's qi blood pain, abdominal pain and other diseases. In recent years, it has been found that HA and its salts possess various pharmacological properties, including anti-inflammatory, antivirus, anti-hypertensive, anti-neoplastic, hemostatic activities. Fulvic acid (FA) is a principal component of HA, which has the smallest molecular weight, highest solubility, strongest biological activity and optimal absorption in vivo, therefore what has been used in medicine is mainly FA. Due to the different degree of coalification and the different extraction method, FA from different area has different pharmacological properties. FA-Na used in this research is made from FA, which is extracted from young lignite of Xundian Yunnan province.The anthraco-propulsion and gastric ulcer model were used to observe effects of FA-Na on the intestine propulsion and gastric ulcer. The hyperlipemia and diabetic mice models were used to observe effects of FA-Na on blood fat and blood glucose.The main results are summarized as follows:1 Using charcoal powder as an indicator, the effects of FA-Na on gastrointestinal motility of mice was evaluated by determining the propulsive percentage of charcoal powder in normal mice, inhibition of gastrointestinal motility model induced by Compound Diphenoxylate and spleen asthenia and diarrhea model caused by Rheum officinale Baill; The protective effect of FA-Na as a prophylactic agent on gastric injury of acute gastric ulcer model induced by intragastric alcohol given orally was evaluated by calculating the ulcer index and the inhibition ratio of ulcer. The experimental results showed that FA-Na (intragastric administration) had some inhibition effects on gastrointestinal motility of normal mice and diarrhea model caused by Rheum officinale Baill, remarkedly synergetic effect on gastrointestinal asynersis induced by Compound Diphenoxylate, and protective effect on mice gastric mucosal injury caused by dehydrated alcohol. Compared with the positive control durg Ranitidine, large doses of FA-Na had no significant differences in ulcer index and ulcer inhibition ratio. The acute toxicity of FA-Na is very low, it has the effects of antidiarrheal and anti-gastric ulcer. 2 Acute high blood fat mice model was induced by intraperitoneal injection of egg yolk. The influences of FA-Na on total cholesterol and triglyceride levels of normal mice and high blood fat mice were researched by detecting the serum total cholesterol and triglyceride levels using the enzyme mark instrument. Results:FA-Na has no significant effects on total cholesterol and triglyceride levels of normal mice and high blood fat mice, therefore FA-Na has no blood lipid lowering effects.3 The diabetic mice model was induced by the intraperitoneal injection of alloxan. The influences of HA-Na, HA-Na, and theabrownine group on blood glucose level, the glucose tolerance, total cholesterol and triglyceride of diabetic mice were studied. Results:The influences of HA-Na, HA-Na, and theabrownine group on blood glucose level, total cholesterol and triglyceride of diabetic mice has no significant difference from that of controlled group. High doses of HA-Na, HA-Na, and theabrownine group can significantly improve the glucose tolerance of diabetic mice.FA-Na possess pharmacological properties of antidiarrheal, anti-gastric ulcer and improving the glucose tolerance of diabetic mice, but it has no significant effects on blood fat level of high blood fat mice and blood glucose level of diabetic mice.