The Effect Of N-Acetylcysteine On The Airway Inflammation And Remodeling Of Obese Asthma Mice

ObjectivesTo evaluate the effect of oxidative stress on the airway inflammation and remodeling of obese asthma mice with high-fat diets,and to explore the effect of N-Acetylcysteine on the airway inflammation and remodeling of obese asthma mice with high-fat diets.MethodsSixty female C57BL/6J mice were randomly divided into 4 groups, asthma group A, obese group B, obese asthma group C and control group D. Group A were sensitized and challenged by ovalbum(OVA) and normal diets, Group B were fed with high-fat diets,while Group C were sensitized, challenged as group A and fed as group B, the Group D were sensitized, challenged with normal saline and fed with normal diets . The cells in BALF were count and classified after staining, IL-6 and 8-iso-ProstaglandinF2α(8-iso-PGF2α) in lung tissue were detected by ELISA. WAt, WAm, Pbm, as the remodeling indices, measured in lung pathological section. All the parameters were compared between 4 groups.MethodsSixty female C57BL/6J mice were randomly divided into 4 groups, asthma group A, obese asthma group B, treatment group C and normal control group D. Group A were sensitized and challenged by ovalbum(OVA) and normal diets, Group B were sensitized and challenged as group A but fed with high-fat diets, while Group C were sensitized, challenged and fed as group B, but administrated N-Acetylcysteine 200mg/kg.d from the third week after challenge. The cells in BALF were count and classified after staining, IL-6 and 8-iso-PGF2αrostaglandin (8-iso-PGF2α) in lung tissue were detected by ELISA. WAt, WAm, Pbm, as the remodeling indices, measured in lung pathological section. All the parameters were compared between 4 groups. ResultsIn Study Iin comparison with group D, the leukocytes and EOS count in BALF, WAt/ Pbm, WAm/Pbm in lung section were increased as well as IL-6 and 8-iso-PGF2αin lung tissue elevated in group A and group C, while the maximum changes were observed in group C(P<0.05). Pearson correlation analysis showed that WAm/Pbm, WAt/ Pbm and IL-6 were in positive correlation with 8-iso-PGF2α(r= 0.785, 0.868, 0.892, P<0.01).In Study IIIn comparison with group D, the leukocytes and EOS count in BALF, WAt/ Pbm, WAm/Pbm in lung section were increased as well as IL-6 and 8-iso-PGF2αin lung tissue elevated in group A and group B, while the maximum changes were observed in group B(P<0.05). After NAC treatment, the IL-6, 8-iso-PGF2αand WAt/ Pbm were decreased significantly (P<0.05). Pearson correlation analysis showed that WAt/ Pbm and IL-6 were in positive correlation with 8-iso-PGF2α(r=0.817, 0.737, P<0.01).ConclusionsOxidative stress was involved in the airway inflammation and remodeling of obese asthma mice with high-fat diets. N-Acetylcysteine can alleviate the airway inflammation and remodeling of asthma by a substantial inhibition of the oxidative stress reaction in lung.