Effects Of VEGF From Pulmonary Sromal Cells On Differentiation And Development Of Dendritic Cells

Dendritic cells (DCs) are not only the most potent antigen-presenting cells (APC),but also can regulate the immune response induct immune tolerance.The organism is complicated system,every composition interact each other at various levels to maintain the steady-state internal environment,then also the immune system.Investigation not confirmed that DCs and HSC can be induced into DCreg during the spleen and liver micro-environment but also expound the mechnisms .Our previous work showed that mDCs can differentiat into DCreg with special phenotype,however the exactly mechnisms wasn't clear.A variety of functional cells, chemokines and cytokines (VEGF, TGF-β, GM-CSF,and PGE2,etc.compose the lung microenvironment.They are the major information-transferor with extremely extensive biologic activity that concert innate immunity with adaptive immunity. We are wondering what's the role of the cytokines in the lung microenvironment? Our research confirms that the VGEF has adjustment function on differentiation, development and function of DCs through effecting the phaenotype and maintain the lung homeostasis.ObjectivesTo establish stable murine pulmonary stmoral cells and observe effects of vascular endothelial growth factor (VEGF)on differentiation, development and function of mature dendritic cells (mDC) and the immune tolerance.MethodsWe established stable murine pulmonary stmoral cells and identified them by morphology and vimentin expression,which is the feature of fibrocyte.Detected murine pulmonary stmoral cells highly secrete cytokines of TGF-β,VEGF and IL-10 by RT-PCR. Established the co-culture system of MPSC/mDC(cello-density:1×106) as control group,then experimental group was added to VEGF-Ab(5μg/ml),both of them were cultivanted one week. The content of cytokines (IL-10 and IL-12p70) and NO in the co-culture supernatants,expression of cellular phenotype and ability of inducing T cells reproductive activity,were detected by enzyme linked immunosorbent assay(ELISA),Griess,fluorescence-activated cell sorting and CCK-8 respectively.The difference between the two groups was then obsereved.ResultsCD86 expression of VEGF-Ab group was higher than control group(P <0.05),however NO was lower than control group(P<0.05).There were no difference of content of IL-10 and IL-12p70 between the two groups(P>0.05).The ability of inducing T cells reproductive activity were both lower than mDCs,but no difference between the two groups(P>0.05).ConclusionsWe established stable murine pulmonary stmoral cells,confirmed that mDC was not the end cell which can differentiate into DCreg within the lung microenvironment and VEGF involved in the procession by reducing expression of costimulatory molecules CD86 and the expression of NO.