Study On Extraction Technics And Quality Control Of Yishen Wufa Syrup

Oral Yishen UFA is a fleece-flower root, Chinese angelica, psoralen, wolfberry fruit, Astragalus complanatus, Poria, Achyranthes composed of seven kinds of Chinese herbal compound preparation, with supplement liver and kidney, Ukraine refund the role. Is mainly used for liver and kidney deficiency caused by the beard and hair off, as early as white. Is closed set in the "ministerial standards" set prescription of Chinese medicine formulations 11 in. At present countries require compound preparation of the medicinal extracts of the preparation process should be further optimized, and their quality should be further control. This topic is in Chinese medicine theory, under the guidance of the application of modern science and technology and methods of Chinese herbal extracts on the kidney UFA Oral Liquid Extraction Process of optimization and validation, and quality standards of systematic research to ensure that the drug preparation of safe and effective, stable and controllable.-Based products to medicinal extracts and preparations provide a basis for quality control.1.The study of extraction methodIn the extraction process study, in order to Fang Jun Stilbene Glucoside from Polygonum multiflorum drug content and extract yield for the evaluation of indicators, examined the increase of water, soaking time, boiling time, boiling effect on the extraction times, using with orthogonal extraction. Ultimately determine the extraction conditions were:Fang Qiwei complementary medicine is about 1.1 times the amount of water absorption capacity, the increase six-fold the amount of 0.5 hours of water soaking, boiling three times with each of 2 hours.2.Herbal extract Study on the Quality StandardBy using the thin-layer chromatography to study the extractions of Achyranthes UFA oral Chinese herbal medicine, Chinese angelica, psoralen, fleece-flower root four herbs flavor were identified, respectively. The results show that test products for chromatography, in comparison with the reference substance or medicinal chromatography corresponding position, substantially the same color of the spots, while the negative control without interference. After numerous experiments, validation, spots clear separation is good, good reproducibility, specificity, this method is the establishment of Chinese herbal medicines for kidney UFA oral flavor extract in the above-mentioned four ingredients provide a basis for qualitative identification.At the same time, using high-performance liquid chromatography of kidney extracts of Chinese medicinal herbs oral UFA stilbene glucoside content. Determination of stilbene glucoside content of the method were as follows:18 alkyl silane bonded silica gel as a filler; Methanol:water (45:55) as mobile phase, column temperature 30℃; detection wavelength 320nm; flow rate lml/min. The results showed that:two stilbene glucoside in the range of 0.11-1.1μg there are a good linear relationship, r=0.9999, recovery was 99.25%, RSD 1.68%, this product per gram of extract containing stilbene glucoside is not less than 2.824mg/g. Determination of study results show that:The method the stability, precision and recovery requirements are in line with quantitative analysis can be used as indicators of quality control of the products.