The Research On Activity Of Lipophilia Non-alkaloid In Ephedra

Objective:Ephedra as a commonly Chinese medicine with complex chemical composition. Components have been reported alkaloids, flavonoids, yellow alkanes, tannins, volatile oils, organic acids, amino acids, polysaccharides and some phenols. At present, the ephedra has been studied broadly in worldwide, but mostly focused on the separation, identification, determination, pharmacology and toxicology in components of alkaloids and volatile oils. It uncommonly to find the research on non-alkaloid and non-volatile oils. Unfortunately, the adverse reactions about chemical composition of alkaloids in ephedra has been reported frequently at domestic and overseas. FDA has banned the use of non-prescription due to the risks of heart disease and stroke. It will bring the adverse effects to the development of ephedra. We study the components of non-alkaloids and non-volatile oils in ephedra. To research the pharmacological activity of these components on experimental hyperlipemia mice, explore the effects of mechanism and physical basis, provide some experimental evidence for development the ephedra.The paper based on Dr. Fan Yanbo' study in the laboratory, lipophilic ephedra non-alkaloid components of acute toxicity was researched, lipophilic ephedra non-alkaloids efficacy studies were reviewed on mouse model of hyperlipidemia made by feeding high fat. Methods:1 lipophilic ephedra non-alkaloids components of acute toxicity: By the Karber method, the male mice,18 to 22g, continued fed for 14 days, was observed animal signs and death in general.2 Mice were fed high fat diet to set up the model of hyperlipidemia. Mice were randomly divided into 6 groups:blank control, model, positive control, lipophilic ephedra non-alkaloids high dose, midlle dose, low dose. Intragastric administration was given continuously for 4 weeks, body weight were recorded weekly. The content of TC, TG, HDL-C and activities of SOD, MDA ALT and AST in serum were measured respectively by semi-automatic biochemical analyzer. The organ coefficient was also calculated. To research the pharmacological activity of this components in the mice of hyperlipidemia.Results:1 In the results no mouse died and no abnormal reaction and The maximum tolerate dose of lipophilic ephedra non-alkaloids small molecules in mice (by body weight) was 60.8 times as the clinical daily dose for adult (50kg), indicating that the component relatively high security.2 Replication experimental hyperlipidemia modelThe mice of TC and TG in serum fed high fat diet were increased obviously indicated experimental hyperlipidemia model replication successful.3 The effects on mice of experimental hyperlipidemia model by lipophilic ephedra non-alkaloidsCompared with the model group, the lipophilic ephedra non-alkaloids markedly decreased the TC, TG and increased the HDL-C in serum (P<0.05 or P<0.01); the lipophilic ephedra non-alkaloids increased the SOD in serum significant and decreased the MDA in serum significant (P<0.05 or P<0.01); the lipophilic ephedra non-alkaloids had no effects on the activities of ALT and AST in serum, lipophilic ephedra non-alkaloids.Conclusion:1 the lipophilic ephedra non-alkaloids had no obvious toxic, basic security2 the experimental hyperlipidemia model was replicated successful.3 the lipophilic ephedra non-alkaloids could improve dyslipidemia on experimental hyperlipidemia model and remove freeradical in serum.