The Study Of Artificial Meshworks Made From Three-dimensional Porous Ceramics In Rabbits

Objective: To evaluate the biocompatibility, safety, efficacy and feasibleness of the artificial meshwork made from three-dimensional porous ceramics after experimental glaucoma surgery in the rabbit.Methods: 11 rabbits were randomly assigned to receive experimental group and normal control. Select one rabbit randomly from 11 healthy adult rabbits, male and female saut, then the rabbit without implanting glaucoma surgery with the artificial meshwork made from three-dimensional porous ceramics was killed as normal control. The other rabbits were performed on implanting glaucoma surgery with the artificial meshwork made from three-dimensional porous ceramics as experimental group.①Observe the formation of filtering blebs and complications on postoperative day 3,7,14,21 and 28.②On postoperative day 3,7,14,21 and 28, measure the intraocular pressure of the rabbits as experimental group and their preoperative intraocular pressure. Then observe the diversity of intraocular pressure.③HE staining and immunohistochemical staining: The sections were observed under light microscope. Observe filtering passages and subconjunctival organization of inflammation all time points by performing HE staining. Immunohistochemistry analysis was to dertermine the expressions and changes of TGF-β1 and bFGF.Results:①There were all functional filtration blebs on postoperative day 3 and 7. Part of functional filtration blebs were disappeared on postoperative day 14 and 21, when the from rates of the functional filtration blebs were 75% and 14%. All the functional filtration blebs were disappeared on postoperative day 28. Light conjunctival congestion, inflammation in the anterior chamber and corneal local edema disappeared after 7 days and had no flat chamber, muddy lens and other complications.②After surgery, the intraocular pressure of experimental group fell. The intraocular pressure was significantly lower on postoperative day 3,7,14 and 21 than the preoperative day. The intraocular pressure was not significantly lower on postoperative day 28. By comparing the intraocular pressure on postoperative adjacent time points, we know that from 14 days or so, intraocular pressure rise faster, and filtration function was reduced obviously.③HE staining : The filtration passages were open on postoperative day 3 and 7. With the extension of time, the filtration passages were filled with collagen, but visible gaps remained on postoperative day 28. The result of the HE staining was consistent with formation conditions of functional filtration blebs. Immunohistochemical staining: The conjunctival epithelial and sclera organizations in the surgical areas showed more obviously positive expressions in experimental group in comparison with normal control group expression, especially on postoperative day 7.Conclusion: The artificial meshworks made from three-dimensional porous ceramics have better biocompatibility and few complications, effectively reduce the intraocular pressure and form functional filtering bleb. Provide a new method for the glaucoma surgery. We still need to further explore production conditions and implantation method of the implantable material. From it's hardness and porosity, we need to research thoroughly, to improve the surgical method, to minimize to reduce the scar tissue, to control the rate of the stream flowing of anterior chamber and to extent the time of functional filtration blebs.